Improving seed germination and oil contents by regulating the GDSL transcriptional level in Brassica napus
Improving seed germination and oil contents by regulating the GDSL transcriptional level in Brassica napus
复制标题
通过调节甘蓝型油菜 GDSL 转录水平提高种子发芽率和含油量
DOI：
10.1007/s00299-018-2365-7
复制
发表时间：
2019-02-01
影响因子：
6.2
通讯作者：
Tan, Xiao-Li
中科院分区：
文献类型：
--
作者：
Ding, Li-Na;Guo, Xiao-Juan;Tan, Xiao-Li
关键信息拟南芥中的AtGDSL 1和BnGDSL 1都可以在转录水平上调控种子萌发率和含油量。摘要Gly-Asp-Ser-Leu（GDSL）基序脂肪酶是脂肪分解酶的一个重要亚家族，在脂类代谢、种子发育、非生物胁迫和病原菌防御中起着重要作用。在本研究中，两个密切相关的GDSL-模体脂肪酶，甘蓝型油菜GDSL 1和拟南芥GDSL 1，其特征在于在B的发芽率和种子油含量的调节功能。油菜。与野生型相比，AtGDSL 1和BnGDSL 1过表达株系的种子发芽率提高，成苗率提高。同时，AtGDSL 1和BnGDSL 1的组成型过表达促进了脂质过氧化，降低了种子含油量。而在B.油菜提高了种子含油量，降低了种子发芽率。此外，Bngdsll转基因种子显示脂肪酸（FA）组成的变化，其特征在于C18：1增加，C18：2和C18：3减少。在AtGDSL 1和BnGDSL 1过表达株系中，参与FA动员的6种相关核心酶的转录水平均升高，但在Bngdsl 1转基因株系中受到强烈抑制。这些结果表明，提高B种子的发芽率和种子含油量。napus中的GDSL基因的转录调控。
Key messageSeed germination rate and oil content can be regulated at theGDSL transcriptional level by eitherAtGDSL1 orBnGDSL1 inB. napus.AbstractGly-Asp-Ser-Leu (GDSL)-motif lipases represent an important subfamily of lipolytic enzymes, which play important roles in lipid metabolism, seed development, abiotic stress, and pathogen defense. In the present study, two closely related GDSL-motif lipases, Brassica napus GDSL1 and Arabidopsis thaliana GDSL1, were characterized as functioning in regulating germination rate and seed oil content in B. napus. AtGDSL1 and BnGDSL1 overexpression lines showed an increased seed germination rate and improved seedling establishment compared with wild type. Meanwhile, the constitutive overexpression of AtGDSL1 and BnGDSL1 promoted lipid catabolism and decreased the seed oil content. While RNAi-mediated suppression of BnGDSL1 (Bngdsl1) in B. napus improved the seed oil content and decreased seed germination rate. Moreover, the Bngdsl1 transgenic seeds showed changes in the fatty acid (FA) composition, featuring an increase in C18:1 and a decrease in C18:2 and C18:3. The transcriptional levels of six related core enzymes involved in FA mobilization were all elevated in the AtGDSL1 and BnGDSL1 overexpression lines, but strongly suppressed in the Bngdsl1 transgenic line. These results suggest that improving the seed germination and seed oil content in B. napus could be achieved by regulating the GDSL transcriptional level.