Entry of Herpes Simplex Virus 1 and Other Alphaherpesviruses via the Paired Immunoglobulin-Like Type 2 Receptor α
Entry of Herpes Simplex Virus 1 and Other Alphaherpesviruses via the Paired Immunoglobulin-Like Type 2 Receptor α
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DOI：
10.1128/jvi.02601-08
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发表时间：
2009-05-01
影响因子：
5.4
通讯作者：
Kawaguchi, Yasushi
中科院分区：
文献类型：
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作者：
Arii, Jun;Uema, Masashi;Kawaguchi, Yasushi
单纯疱疹病毒1型（HSV-1）通过病毒粒子包膜和宿主细胞质膜的融合或通过内吞作用进入细胞，具体取决于细胞类型。在这里报道的研究中，我们研究了依赖于成对免疫球蛋白样2型受体α（PILR α）的病毒进入途径，PILR α是最近鉴定的HSV-1的进入辅助受体，与病毒包膜糖蛋白B（gB）相关。使用内吞途径抑制剂的实验和用PILR α转导的中国仓鼠卵巢（CHO）细胞的超微结构分析表明，HSV-1通过细胞表面的病毒-细胞融合进入这些细胞。与早期观察到的HSV-1摄取进入正常CHO细胞和用HSV-1包膜gD受体转导的那些细胞是由内吞作用介导的一起，这些结果表明PILR α的表达在CHO细胞中产生了替代的HSV-1进入途径。我们还表明，人类和小鼠PILR α能够介导伪狂犬病病毒（一种猪α疱疹病毒）的进入，但不能介导HSV-2的进入。这些结果表明，病毒通过PILR α进入似乎是保守的，但在α疱疹病毒中存在PILR α偏好。
Herpes simplex virus 1 (HSV-1) enters cells either via fusion of the virion envelope and host cell plasma membrane or via endocytosis, depending on the cell type. In the study reported here, we investigated a viral entry pathway dependent on the paired immunoglobulin-like type 2 receptor alpha (PILR alpha), a recently identified entry coreceptor for HSV-1 that associates with viral envelope glycoprotein B (gB). Experiments using inhibitors of endocytic pathways and ultrastructural analyses of Chinese hamster ovary (CHO) cells transduced with PILR alpha showed that HSV-1 entry into these cells was via virus-cell fusion at the cell surface. Together with earlier observations that HSV-1 uptake into normal CHO cells and those transduced with a receptor for HSV-1 envelope gD is mediated by endocytosis, these results indicated that expression of PILR alpha produced an alternative HSV-1 entry pathway in CHO cells. We also showed that human and murine PILR alpha were able to mediate entry of pseudorabies virus, a porcine alphaherpesvirus, but not of HSV-2. These results indicated that viral entry via PILR alpha appears to be conserved but that there is a PILR alpha preference among alphaherpesviruses.