A microfluidic system for dynamic yeast cell imaging
A microfluidic system for dynamic yeast cell imaging
复制标题
DOI：
10.2144/000112673
复制
发表时间：
2008-01-01
期刊：
影响因子：
2.7
通讯作者：
Hung, Paul J.
中科院分区：
文献类型：
--
作者：
Lee, Philip J.;Helman, Noah C.;Hung, Paul J.
对活细胞内的细胞过程和基因调控网络的研究需要开发改进的动态单细胞成像技术。在这里，我们展示了一种微流体系统，能够在延时高倍荧光成像期间机械捕获具有连续流动和流动切换能力的酵母细胞。通过观察酿酒酵母中信息素诱导的 GFP 表达的反应，验证了该系统的新功能。
The investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching capability during time-lapse high magnification fluorescence imaging. The novel functionality of the system was validated by observing the response of pheromone-induced expression of GFP in Saccharomyces cerevisiae.