Characterisation of adherens and tight junctional molecules in normal animal larynx; Determining a suitable model for studying molecular abnormalities in human laryngopharyngeal reflux
Characterisation of adherens and tight junctional molecules in normal animal larynx; Determining a suitable model for studying molecular abnormalities in human laryngopharyngeal reflux
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DOI：
10.1136/jcp.2004.016972
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发表时间：
2005-12-01
影响因子：
3.4
通讯作者：
Pignatelli, M
中科院分区：
文献类型：
--
作者：
Gill, GA;Buda, A;Pignatelli, M
背景：喉部细胞间连接的破坏是喉咽反流（LPR）的病理特征。为了更深入地了解喉上皮异常暴露于胃酸反流而导致的分子机制和变化，需要良好的实验模型。目的：表征不同物种的喉组织，以确定最适合用于 LPR 实验研究的组织。方法：对人类和非人类喉组织（小鼠、大鼠、豚鼠、猪和兔子）进行研究。通过光学显微镜进行组织学表征。通过免疫组织化学评估粘附连接分子（E-钙粘蛋白和β连环蛋白）的表达和亚细胞定位，并通过蛋白质印迹评估紧密连接分子（occludin和闭合小带1（ZO-1））的表达和亚细胞定位。通过电子显微镜评估猪和人体组织的超微结构特征。结果：猪组织显示呼吸型和复层鳞状上皮，如人喉部所见。除小鼠和大鼠外，所有物种均检测到 E-钙粘蛋白-连环蛋白复合物的表达和亚细胞定位。所有物种均保留了ZO-1和occludin的表达模式。结论：猪上皮细胞间连接复合物的表达与人类相似。这些结果证实了这些物种适合在实验系统中研究 LPR 的分子机制。
Background: The disruption of intercellular junctions in the larynx is a pathological feature of laryngopharyngeal reflux (LPR). Good experimental models are necessary to gain greater insight into the molecular mechanisms and alterations that result from abnormal exposure of the laryngeal epithelium to acid refluxate.Aims: To characterise laryngeal tissues from different species to determine the most suitable for use in experimental studies of LPR.Methods: Human and non-human laryngeal tissues (mouse, rat, guinea pig, porcine, and rabbit) were studied. Histological characterisation was performed by light microscopy. The expression and subcellular localisation of adherens junctional molecules (E-cadherin and beta catenin) was evaluated by immunohistochemistry, and tight junction molecules (occludin and zonula occludens 1 ( ZO-1)) by western blotting. The ultrastructural features of porcine and human tissue were assessed by electron microscopy.Results: Porcine tissue revealed both respiratory-type and stratified squamous epithelium, as seen in the human larynx. The expression and subcellular localisation of the E-cadherin-catenin complex was detected in all species except mouse and rat. The pattern of ZO-1 and occludin expression was preserved in all species.Conclusion: The expression of intercellular junctional complexes in porcine epithelium is similar to that seen in humans. These results confirm the suitability of these species to study molecular mechanisms of LPR in an experimental system.