The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis.
The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis.
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DOI：
10.1111/j.1469-8137.2012.04339.x
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发表时间：
2012-12
期刊：
影响因子：
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通讯作者：
S. Yoshida;H. Kameoka;M. Tempo;K. Akiyama;Mikihisa Umehara;Shinjiro Yamaguchi;H. Hayashi;J. Kyozuka;K. Shirasu
中科院分区：
文献类型：
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作者：
S. Yoshida;H. Kameoka;M. Tempo;K. Akiyama;Mikihisa Umehara;Shinjiro Yamaguchi;H. Hayashi;J. Kyozuka;K. Shirasu
丛枝菌根（AM）是一种古老的植物根系与球囊霉门真菌的内共生关系。独脚金内酯（SL），植物源萜类内酯，激活AM真菌的菌丝分支之前，物理接触。SL生物合成的缺乏导致AM真菌的定殖较低。F-box蛋白DWARF 3（D3）和水解酶家族蛋白DWARF 14（D14）对水稻的SL反应至关重要。在这里，我们进行了AM真菌定殖测定SL-不敏感的d3和d14突变体。d3突变体表现出强烈的缺陷，在AM真菌定植，而d14突变体表现出较高的AM真菌定植。由于D14具有同源蛋白D14-LIKE，我们通过在野生型和d14背景中的RNA干扰产生D14-LIKE敲低系。D14和D14-LIKE双敲低系表现出与d14-1突变体相似的定殖率。D3是水稻AM真菌共生的关键因子，而D14和D14-LIKE则不是。我们的研究结果表明，这些SL相关的组件AM共生的独特作用。
Arbuscular mycorrhiza (AM) represents an ancient endosymbiosis between plant roots and Glomeromycota fungi. Strigolactones (SLs), plant-derived terpenoid lactones, activate hyphal branching of AM fungi before physical contact. Lack of SL biosynthesis results in lower colonization of AM fungi. The F-box protein, DWARF3 (D3), and the hydrolase family protein DWARF14 (D14) are crucial for SL responses in rice. Here we conducted AM fungal colonization assays with the SL-insensitive d3 and d14 mutants. The d3 mutant exhibited strong defects in AM fungal colonization, whereas the d14 mutant showed higher AM fungal colonization. As D14 has a homologous protein, D14-LIKE, we generated D14-LIKE knockdown lines by RNA interference in the wildtype and d14 background. D14 and D14-LIKE double knockdown lines exhibited similar colonization rates as those of the d14-1 mutant. D3 is crucial for establishing AM symbiosis in rice, whereas D14 and D14-LIKE are not. Our results suggest distinct roles for these SL-related components in AM symbiosis.