Surface Modification of Electrospun Polycaprolactone Nanofiber Meshes by Plasma Treatment to Enhance Biological Performance
Surface Modification of Electrospun Polycaprolactone Nanofiber Meshes by Plasma Treatment to Enhance Biological Performance
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DOI：
10.1002/smll.200801648
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发表时间：
2009-05-18
期刊：
影响因子：
13.3
通讯作者：
Neves, Nuno M.
中科院分区：
文献类型：
--
作者：
Martins, Albino;Pinho, Elisabete D.;Neves, Nuno M.
生物材料开发的一个关键方面是优化其表面特性，以实现适当的细胞反应。在本工作中，静电纺丝聚己内酯纤维网（NFM）的射频（RF）等离子体处理使用不同的气体（Ar或O-2），功率（20或30 W），和曝光时间（5或10分钟）。形态学和粗糙度分析显示等离子体处理的NFM上的地形变化。X射线光电子能谱（XPS）的结果表明，含氧基团，主要是-OH和-C=O，在等离子体处理的表面的增量。因此，甘油接触角结果表明等离子体处理补片的疏水性降低，尤其是O-2处理补片。使用三种模型细胞系（成纤维细胞、软骨细胞和成骨细胞）研究等离子体处理对形态、细胞粘附和增殖的影响。发现用O2的等离子体处理和用Ar的等离子体处理对于所有研究的细胞类型是最成功的。讨论了亲水性和粗糙度对纳米纤维膜生物学性能的影响。尽管经常声称NFM与天然细胞外基质的形态相似性，但它们的表面性质对细胞性能有很大贡献，因此应该优化它们。
A critical aspect in the development of biomaterials is the optimization of their surface properties to achieve an adequate cell response. In the present work, electrospun polycaprolactone nanofiber meshes (NFMs) are treated by radio-frequency (RF) plasma using different gases (Ar or O-2), power (20 or 30 W), and exposure time (5 or 10 min). Morphological and roughness analysis show topographical changes on the plasma-treated NFMs. X-ray photoelectron spectroscopy (XPS) results indicate an increment of the oxygen-containing groups, mainly -OH and -C=O, at the plasma-treated surfaces. Accordingly, the glycerol contact angle results demonstrate a decrease in the hydrophobicity of plasma-treated meshes, particularly in the O-2-treated ones. Three model cell lines (fibroblasts, chondrocytes, and osteoblasts) are used to study the effect of plasma treatments over the morphology, cell adhesion, and proliferation. A plasma treatment with 02 and one with Ar are found to be the most successful for all the studied cell types. The influence of hydrophilicity and roughness of those NFMs on their biological performance is discussed. Despite the often claimed morphological similarity of NFMs to natural extracellular matrixes, their surface properties contribute substantially to the cellular performance and therefore those should be optimized.