Panax Notoginseng Saponins Ameliorates Coxsackievirus B3-Induced Myocarditis by Activating the Cystathionine-γ-Lyase/Hydrogen Sulfide Pathway.
Panax Notoginseng Saponins Ameliorates Coxsackievirus B3-Induced Myocarditis by Activating the Cystathionine-γ-Lyase/Hydrogen Sulfide Pathway.
复制标题
Panax notoginseng saponins通过激活Cystathionine-γ-裂解酶/硫化氢途径来改善Coxsackievivirus B3诱导的心肌炎。
DOI：
10.1007/s12265-015-9659-8
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发表时间：
2015-12
影响因子：
3.4
通讯作者：
Zhang C
中科院分区：
文献类型：
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作者：
Pan L;Zhang Y;Lu J;Geng Z;Jia L;Rong X;Wang Z;Zhao Q;Wu R;Chu M;Zhang C
本研究旨在探讨三七总皂苷（PNS）对柯萨奇病毒B3（CVB 3）诱导的心肌炎的治疗作用及其与胱硫醚-γ-裂解酶（CSE）/硫化氢（H2S）通路的关系。通过CVB 3感染诱导小鼠心肌炎模型，并使小鼠经受媒介物（盐水）或药物治疗（硫氢化钠（NaHS）、炔丙基甘氨酸（PAG）或PNS）。结果表明，CVB 3诱导的心肌炎存在炎性细胞浸润、间质水肿及炎性细胞因子升高。PAG给药增加，而NaHS治疗降低了心肌炎的严重程度。三七总皂苷可明显减轻心肌细胞损伤，并通过增强CSE/H2S通路的表达，降低病毒mRNA的表达。PNS对心肌炎的治疗作用强于NaHS。最后，在培养的心肌细胞中验证了PNS对CSE/H2S通路的影响和心肌细胞保护作用。三七总皂苷有望成为治疗病毒性心肌炎的有效药物。
This study is to determine the therapeutic effects of Panax notoginseng saponins (PNS) on coxsackievirus B3 (CVB3)-induced myocarditis, and whether cystathionine-γ-lyase (CSE)/hydrogen sulfide (H2S) pathway is involved. Mouse model of myocarditis was induced by CVB3 infection and the mice were subjected to vehicle (saline) or drug treatments (sodium bisulfide (NaHS), propargylglycine (PAG) or PNS). The results showed that there were inflammatory cell infiltrations, interstitial edemas, as well as elevated inflammatory cytokines, in CVB3-induced myocarditis. PAG administration increased, whereas NaHS treatment decreased the severity of the myocarditis. PNS treatment dramatically alleviated these myocardial injuries and decreased the viral mRNA expression by the enhanced expression of CSE/H2S pathway. Moreover, the therapeutic effects of PNS on myocarditis were stronger than NaHS. Finally, the effect of PNS on CSE/H2S pathway and cardiac cell protection were verified in cultured cardiac cells. PNS may be a promising medication for viral myocarditis therapy.