Global distribution of epidemic-related Shiga toxin 2 encoding phages among enteroaggregative Escherichia coli
Global distribution of epidemic-related Shiga toxin 2 encoding phages among enteroaggregative Escherichia coli
复制标题
流行相关志贺毒素2编码噬菌体在肠聚集性大肠杆菌中的全球分布
DOI：
10.1038/s41598-020-68462-9
复制
发表时间：
2020-07-16
影响因子：
4.6
通讯作者：
Iyoda, Sunao
中科院分区：
文献类型：
--
作者：
Kimata, Keiko;Lee, Kenichi;Iyoda, Sunao
自2011年产滋贺毒素肠聚集性大肠杆菌（Stx-EAEC）O 104：H4菌株在欧洲引起大规模爆发以来，Stx-EAEC的重要性从公共卫生角度引起了关注。1999年和2015年，从日本的重度症状患者中获得了两株Stx-EAEC O 86分离株。为了表征这些Stx-EAEC O 86分离株的致病性和致病潜力，通过短读和长读测序进行全基因组序列分析。在遗传多样性E. coli O 86，与EAEC O 86：H27 ST 3570亚群聚为一类。引人注目的是，只有两个基因座之间的单核苷酸多态性（SNP）的Stx 2a噬菌体的日本O 86：H27分离株和欧洲的沙门氏菌相关的Stx-EAEC O 104：H4分离株。这些结果提供了证据，全球分布的寄生虫相关的Stx 2a的各种谱系的E。大肠杆菌中有少量突变。
Since the Shiga toxin-producing enteroaggregative Escherichia coli (Stx-EAEC) O104:H4 strain caused a massive outbreak across Europe in 2011, the importance of Stx-EAEC has attracted attention from a public health perspective. Two Stx-EAEC O86 isolates were obtained from patients with severe symptoms in Japan in 1999 and 2015. To characterize the phylogeny and pathogenic potential of these Stx-EAEC O86 isolates, whole-genome sequence analyses were performed by short-and long-read sequencing. Among genetically diverse E. coli O86, the Stx-EAEC O86 isolates were clustered with the EAEC O86:H27 ST3570 subgroup. Strikingly, there were only two loci with single nucleotide polymorphisms (SNPs) between the Stx2a phage of a Japanese O86:H27 isolate and that of the European epidemic-related Stx-EAEC O104:H4 isolate. These results provide evidence of global distribution of epidemic-related Stx2a phages among various lineages of E. coli with few mutations.