Enzymatic Resistance of Corneas Crosslinked Using Riboflavin in Conjunction With Low Energy, High Energy, and Pulsed UVA Irradiation Modes.
Enzymatic Resistance of Corneas Crosslinked Using Riboflavin in Conjunction With Low Energy, High Energy, and Pulsed UVA Irradiation Modes.
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DOI：
10.1167/iovs.15-18769
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发表时间：
2016-04
影响因子：
4.4
通讯作者：
Meek KM
中科院分区：
文献类型：
--
作者：
Aldahlawi NH;Hayes S;O'Brart DP;Akhbanbetova A;Littlechild SL;Meek KM
研究不同核黄素/紫外线（UVA）交联（CXL）方案对角膜酶抗性的影响。将66只摘除角膜上皮的猪眼分为6组。第1组未接受治疗。第2至6组接受核黄素/葡聚糖30分钟。第3组接受标准CXL（SCXL），UVA为3 mW/cm 2，持续30分钟（总能量剂量为5.4 J/cm 2）。第4组和第5组分别使用30 mW/cm 2 UVA（5.4 J/cm 2）和30 mW/cm 2 UVA（7.2 J/cm 2）进行高强度CXL（HCXL）治疗3分钟和4分钟。第6组以10秒开/10秒关脉冲辐射模式（p-HCXL; 7.2 J/cm 2）暴露于30 mW/cm 2 UVA 8分钟。将来自每个角膜的中心8 mm圆盘浸没在23 ° C的胃蛋白酶消化溶液中，并每天测量。13天后，记录每组5个样品的干重。CXL处理的角膜比未照射的角膜消化时间更长（P <0.0001）。CXL组之间的消化时间也存在差异，HCXL（5.4 J/cm 2）<SCXL（5.4 J/cm 2）<HCXL（7.2 J/cm 2）<p-HCXL（7.2 J/cm 2; P <0.0001）。SCXL（5.4 J/cm 2）组的干重高于HCXL（5.4和7.2 J/cm 2; P <0.001）和p-HCXL 7.2 J/cm 2（P <0.05）组。HCXL和p-HCXL 7.2 J/cm2组之间未检测到差异。角膜内形成的交联的强度和分布随不同的UVA方案而变化。预防疾病进展所需的精确位置和交联量尚不清楚。
To investigate the effect of various riboflavin/ultraviolet light (UVA) crosslinking (CXL) protocols on corneal enzymatic resistance. A total of 66 enucleated porcine eyes, with the corneal epithelium removed, were divided into 6 groups. Group 1 remained untreated. Groups 2 to 6 received riboflavin/dextran for 30 minutes. Group 3 underwent standard CXL (SCXL) with 3 mW/cm2 UVA for 30 minutes (total energy dose 5.4 J/cm2). Groups 4 and 5 underwent high intensity CXL (HCXL) using 30 mW/cm2 UVA for 3 minutes (5.4 J/cm2) and 30 mW/cm2 for 4 minutes (7.2 J/cm2), respectively. Group 6 was exposed to 8 minutes of 30 mW/cm2 UVA in a 10-second on/10-second off pulsed-radiation mode (p-HCXL; 7.2 J/cm2). A central 8-mm disk from each cornea was submerged in pepsin digest solution at 23°C and measured daily. After 13 days, the dry weight was recorded from 5 samples in each group. The CXL-treated corneas took longer to digest than nonirradiated corneas (P < 0.0001). Differences in digestion time also were observed between CXL groups, such that, HCXL (5.4 J/cm2) < SCXL (5.4 J/cm2) < HCXL (7.2 J/cm2) < p-HCXL (7.2 J/cm2; P < 0.0001). The dry weight of the SCXL (5.4 J/cm2) group was higher than the HCXL (5.4 and 7.2 J/cm2; P < 0.001) and p-HCXL 7.2 J/cm2 (P <0.05) groups. No difference was detected between the HCXL and p-HCXL 7.2 J/cm2 groups. The intensity and distribution of the crosslinks formed within the cornea vary with different UVA protocols. The precise location and amount of crosslinking needed to prevent disease progression is unknown.