Novel synthesis of gold nanoclusters templated with L-tyrosine for selective analyzing tyrosinase.
Novel synthesis of gold nanoclusters templated with L-tyrosine for selective analyzing tyrosinase.
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DOI：
10.1016/j.aca.2014.05.050
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发表时间：
2014-08
影响因子：
6.2
通讯作者：
Xiaoming Yang;Yawen Luo;Yan Zhuo;Yuanjiao Feng;Shanshan Zhu
中科院分区：
文献类型：
--
作者：
Xiaoming Yang;Yawen Luo;Yan Zhuo;Yuanjiao Feng;Shanshan Zhu
l-Tyrosine (Tyr), playing roles as both a reducing reagent and a protecting ligand, has been first employed for synthesizing fluorescent gold nanoclusters (AuNCs@Tyr) via a novel one-pot strategy. The as-prepared AuNCs@Tyr exhibited a fluorescence emission at 470 nm with a quantum yield of approximately 2.5%. Subsequently, the AuNCs@Tyr described here was applied for detections of tyrosinase (TR) activity, which was based on the mechanism of aggregations of AuNCs@Tyr occurring on the active sites of TR since TR was introduced, thus leading to the fluorescence quenching of AuNCs@Tyr. Accordingly, TR was analyzed in a linear range of 0.5–200 u mL−1with a detection limit of 0.08 u mL−1at a signal-to-noise ratio of 3. Significantly, TR has been considered as a critical marker for melanoma owing to its specifically expressing in melanoma cells. Therefore, this analytical method towards investigating TR activity may broaden avenues for meaningfully clinical applications.
