Clearance kinetics and fate of mouse IgA immune complexes prepared with monomeric or dimeric IgA.
Clearance kinetics and fate of mouse IgA immune complexes prepared with monomeric or dimeric IgA.
复制标题
DOI：
10.4049/jimmunol.130.4.1826
复制
发表时间：
1983-04
影响因子：
4.4
通讯作者：
A. Rifai;M. Mannik
中科院分区：
文献类型：
--
作者：
A. Rifai;M. Mannik
为了确定实验性 IgA 肾病的病理生理机制，研究了小鼠中可溶性 IgA 免疫复合物的清除动力学和组织分布。从 MOPC-315 获得的纯化放射性标记二聚体 (dIgA) 和单体 (mIgA) IgA 抗二硝基苯基与二价亲和标记抗原双-2,4-二硝基苯基庚二酸酯共价交联。静脉注射后注射后，重聚合物（大于 1.2 X 10(6) m.w.）迅速从循环中去除。通过梯度聚丙烯酰胺凝胶电泳对循环中间晶格复合物的分析表明，快速消除需要具有最小组成为 4 dIgA 或 8 mIgA 的聚合物。晶格小于该临界尺寸的 dIgA 和 mIgA 复合物以较慢的速率去除（对于具有 dIgA 的复合物，产生 35 分钟的 t1/2；对于具有 mIgA 的复合物，产生 60 分钟的 t1/2）。 dIgA 和 mIgA 免疫复合物的组织分布相似。肝脏是参与摄取 IgA 免疫复合物的主要器官，胆汁中的量微不足道。 dIgA 或 mIgA 的重聚合物主要位于肝非实质细胞中。
To determine the pathophysiologic mechanism(s) involved in experimental IgA nephropathy, the clearance kinetics and tissue distribution of soluble IgA immune complexes in mice were investigated. Purified radiolabeled dimeric (dIgA) and monomeric (mIgA) IgA antidinitrophenyl, obtained from MOPC-315, were covalently cross-linked with a bivalent affinity-labeling antigen, bis-2,4-dinitrophenyl pimelic ester. After i.v. injection, heavy polymers (greater than 1.2 X 10(6) m.w.) were rapidly removed from circulation. Analysis of circulating intermediate-latticed complexes by gradient polyacrylamide gel electrophoresis indicated that polymers with a minimal composition of four dIgA or eight mIgA were required for rapid elimination. The dIgA and mIgA complexes with lattices smaller than this critical size were removed at slower rates (yielding a t1/2 of 35 min for complexes with dIgA and a t1/2 of 60 min for complexes with mIgA). Tissue distribution of both dIgA and mIgA immune complexes was similar. The liver was the major organ involved in uptake of IgA immune complexes with an insignificant amount in the bile. Heavy polymers of dIgA or mIgA were predominantly localized in the hepatic nonparenchymal cells.