Upregulation of the secretory-type Na(+)/K(+)/2Cl(-)-cotransporter in the kidney by metabolic acidosis and dehydration in rats.
Upregulation of the secretory-type Na(+)/K(+)/2Cl(-)-cotransporter in the kidney by metabolic acidosis and dehydration in rats.
复制标题
大鼠代谢性酸中毒和脱水导致肾脏中分泌型 Na( )/K( )/2Cl(-) 协同转运蛋白的上调。
DOI：
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发表时间：
2001
影响因子：
13.6
通讯作者：
K. Tomita
中科院分区：
文献类型：
--
作者：
M. Ikebe;H. Nonoguchi;Y. Nakayama;Y. Tashima;K. Tomita
Na(+)/K(+)/2Cl(-)-共转运体NKCC1在肾脏中的功能作用和调控机制尚不清楚。采用逆转录- pcr和Western blotting检测对照大鼠、脱水大鼠（2 d）和代谢性酸中毒大鼠（6 ~ 7 d） NKCC1 mRNA和蛋白的表达。NKCC1 mRNA在小鼠终末髓内收集管中表达丰富，而在大鼠髓外收集管（OMCD）中表达最丰富。脱水和代谢性酸中毒不仅使OMCD中NKCC1 mRNA的表达增加了3 - 5倍，而且在皮质集束管和髓内集束管中也增加了3 - 5倍。脱水和代谢性酸中毒使外髓质膜组分中NKCC1蛋白的表达增加两倍。NKCC1蛋白的表达不存在于微解剖的髓质厚升肢中，而存在于OMCD中，并受到脱水和代谢性酸中毒的双重刺激。OMCD在低ph培养基中培养可提高NKCC1 mRNA的表达。综上所述，NKCC1 mRNA和蛋白的表达随着脱水和代谢性酸中毒而上调。NKCC1可能在适应这些生理条件中发挥重要作用。低pH和可能的高渗刺激了OMCD中NKCC1 mRNA的表达。
The functional role and mechanisms of regulation of the Na(+)/K(+)/2Cl(-)-cotransporter NKCC1 in the kidney have not yet been clarified. NKCC1 mRNA and protein expression in control rats, rats with dehydration (2 d), and rats with metabolic acidosis (NH(4)Cl in the food for 6 to 7 d) was examined using reverse transcription-PCR and Western blotting. In contrast to the abundant NKCC1 mRNA expression in the terminal inner medullary collecting ducts in mice, expression was found to be most abundant in the outer medullary collecting ducts (OMCD) in rats. Dehydration and metabolic acidosis increased NKCC1 mRNA expression three- to fivefold not only in the OMCD but also in the cortical collecting ducts and inner medullary collecting ducts. Dehydration and metabolic acidosis increased NKCC1 protein expression twofold in the membrane fraction from the outer medulla. NKCC1 protein expression was observed not in the microdissected medullary thick ascending limbs but in the OMCD, and it was stimulated twofold by dehydration and metabolic acidosis. Incubation of OMCD in low-pH medium increased NKCC1 mRNA expression. In summary, NKCC1 mRNA and protein expression is upregulated with dehydration and metabolic acidosis. NKCC1 may play an important role in adaptation to these physiologic conditions. Low pH and possibly hypertonicity stimulate NKCC1 mRNA expression in OMCD.
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DOI：
10.1172/jci118844
发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
10.1152/ajprenal.1996.271.4.f931
发表时间：
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期刊：
The American journal of physiology.
影响因子：
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DOI：
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发表时间：
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影响因子：
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影响因子：
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DOI：
10.1681/asn.v7122533
发表时间：
1996
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
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作者：
Ginns,SM;Knepper,MA;Ecelbarger,CA;Terris,J;He,X;Coleman,RA;Wade,JB
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Wade,JB