Control of mandibular gland pheromone synthesis by alternative splicing of the CP-2 transcription factor gemini in honeybees (Apis mellifera carnica)
Control of mandibular gland pheromone synthesis by alternative splicing of the CP-2 transcription factor gemini in honeybees (Apis mellifera carnica)
复制标题
DOI：
10.1007/s13592-018-0571-5
复制
发表时间：
2018-03
期刊：
影响因子：
2.4
通讯作者：
Antje Jarosch-Perlow;A. Yusuf;C. Pirk;R. Crewe;R. Moritz
中科院分区：
文献类型：
--
作者：
Antje Jarosch-Perlow;A. Yusuf;C. Pirk;R. Crewe;R. Moritz
蜂王下颌腺信息素（QMP）是抑制工蜂繁殖所必需的信息素。工蜂卵巢的激活受一个名为gemini的CP2转录因子的选择性剪接调控。由于具有激活卵巢的工人也在其下颌腺中产生QMP，我们使用RNA干扰测试了选择性剪接ofgemini是否也控制工人下颌腺信息素的生物合成。改变剪接模式ofgemini导致增强水平的女王特定组件的下颌腺信息素在无女王蜜蜂工人，这表明thegemini功能作为一个多效性调节开关，影响卵巢激活，并导致QMP合成的工人。由于这些工人产生的QMP抑制卵巢激活在其他工人，geminiseems是一个关键的调节基因，影响无女王殖民地的工人之间的生殖层次。
The honeybee queen’s mandibular gland pheromones (QMP) are essential for the suppression of worker reproduction. Worker ovary activation is regulated by alternative splicing of a CP2-transcription factor namedgemini. Since workers with activated ovaries also produce QMP in their mandibular glands, we tested whether alternative splicing ofgeminialso controls mandibular gland pheromone biosynthesis in workers using RNA interference. Altering the splice pattern ofgeminiresulted in enhanced levels of the queen-specific components of the mandibular gland pheromone in queenless honeybee workers, suggesting thatgeminifunctions as a pleiotropic regulatory switch influencing both ovary activation and resulting in QMP synthesis in workers. Because the QMP produced by these workers suppresses ovary activation in other workers,geminiseems to be a key regulatory gene affecting reproductive hierarchies among workers in queenless colonies.