Induction of Ia and H-2 antigens on a macrophage cell line by immune interferon.
Induction of Ia and H-2 antigens on a macrophage cell line by immune interferon.
复制标题
免疫干扰素在巨噬细胞系上诱导 Ia 和 H-2 抗原。
作者：
D. King;P. Jones
最近一些实验室的工作表明，巨噬细胞上Ia抗原的表达可以受到T细胞在抗原或有丝分裂原激活后释放的可溶性因子的调节。小鼠巨噬细胞肿瘤细胞系WEHI-3为研究这种类型的淋巴因子对Ia抗原表达的调节提供了一个体外系统。我们实验室以前的工作表明，刀豆蛋白A激活的大鼠或小鼠脾细胞的上清(ConA Sup)可以刺激该细胞系上Ia和H-2K，D抗原的生物合成和细胞表面表达增加。本报告中描述的实验旨在确定导致这种诱导活性的调节因子，特别是确定粗上清液中存在的诱导因子是否是免疫干扰素(干扰素-γ)。这些实验表明：1)通过重组DNA技术和DNA介导的基因转移产生的不含其他淋巴因子的小鼠干扰素-γ，可诱导WEHI-3细胞表达Ia抗原，并增加H-2抗原的表达；2)从粗提液中部分纯化的诱导因子的生化特征与其他研究人员先前对小鼠干扰素-γ的描述相似；以及3)Con-A粗提物和含干扰素-γ的上清均具有诱导活性和抗病毒活性，且呈现出非常相似的剂量相关反应。这些结果支持了ConA sup调节WEHI-3细胞MHC抗原表达的能力是由于这些细胞上清液中免疫干扰素的活性所致。
Recent work from a number of laboratories has suggested that Ia antigen expression on macrophages can be modulated by soluble factors released by T cells after antigen or mitogen activation. The murine macrophage tumor cell line, WEHI-3, provides an in vitro system for the study of this type of lymphokine regulation of Ia antigen expression. Previous work from our laboratory has shown that supernates from Concanavalin A-activated rat or mouse spleen cells (Con A sup) can stimulate increased levels of biosynthesis and cell surface expression of both Ia and H-2K,D antigens on this cell line. The experiments described in this report were designed to identify the regulatory factor responsible for this inducing activity, and in particular to determine whether the inducing factor present in crude supernatant fluids is immune interferon (IFN-gamma). These experiments show that: 1) murine IFN-gamma, produced free of other lymphokines by recombinant DNA technology and DNA-mediated gene transfer, can induce Ia antigen expression and increase H-2 antigen expression on WEHI-3 cells; 2) the biochemical characteristics of partially purified inducing factor from crude supernate fluids are similar to those previously described for murine IFN-gamma by other investigators; and 3) both crude Con A sup and IFN-gamma-containing supernates have inducing activity and antiviral activity which exhibit very similar dose-related responses. These results support the conclusion that the previously described ability of Con A sup to regulate MHC antigen expression on WEHI-3 cells is due to the activity of immune interferon in those supernates.