Differential gene expression of livers from ApoE deficient mice
Differential gene expression of livers from ApoE deficient mice
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DOI：
10.1016/s0024-3205(00)00912-7
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发表时间：
2000-11-24
期刊：
影响因子：
6.1
通讯作者：
Liu, YC
中科院分区：
文献类型：
--
作者：
Huang, GS;Yang, SM;Liu, YC
使用含有约9，000个人cDNA克隆的人cDNA微阵列对ApoE缺陷小鼠的肝脏进行差异表达基因的基因组调查。由于小鼠和人之间的同源性，杂交在较低严格条件下进行，比常规杂交温度低10度。在基因组水平上产生与高水平血液胆固醇相对应的肝脏基因表达谱。从差异表达基因库中随机选取37个基因进行半定量RT-PCR，进一步验证微阵列杂交的结果。这些包括与动脉粥样硬化相关的基因，以及暗示与高水平血液胆固醇相关的新途径的新基因。人类基因芯片是目前所有物种中保存最完整的基因芯片，它的快速性和准确性为研究其他哺乳动物系统提供了新的思路。(C)2000 Elsevier Science Inc. All rights reserved.
A genomic survey for differentially expressed genes was performed to livers of ApoE deficient mice using human cDNA microarray containing approximately 9,000 human cDNA clones. Due to the homology between mouse and human, hybridization was performed at lower stringency condition, 10 degrees below the regular hybridizing temperature. Gene expression profiles of livers corresponding to high levels of blood cholesterol were generated at genomic scale. Thirty-seven genes were randomly selected from a pool of differentially expressed genes and subjected to semi-quantitative RT-PCR, further confirmed the result from microarray hybridization. These included genes associated with atherosclerosis, and novel genes that implied novel pathways correlated to high levels of blood cholesterol. It is promising using human cDNA microarray, the most complete collection among all species, to study other mammalian systems with satisfying speed and accuracy. (C) 2000 Elsevier Science Inc. All rights reserved.