DETERMINATION OF COPPER(II) CHLOROPHYLLIN BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
DETERMINATION OF COPPER(II) CHLOROPHYLLIN BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/0021-9673(94)80315-3
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发表时间：
1994-09-09
影响因子：
4.1
通讯作者：
LI, S
中科院分区：
文献类型：
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作者：
INOUE, H;YAMASHITA, H;LI, S
以脱镁叶绿酸铜（II）a、二氢卟酚铜（II）e（6）、罗丹明铜（II）g（7）和二氢卟酚铜（II）e（4）为原料，制备了叶绿酸铜（II）。采用Inertsil ODS-2反相色谱柱，以甲醇-水（97：3，v/v）（含1%乙酸）为移动的流动相，测定了叶绿素铜（II）的组成。在0-30 μ g cm（-3）浓度范围内，用407或423 nm的光度检测得到叶绿素铜（II）的线性校准图。铜（Ⅱ）脱镁叶绿酸a、铜（Ⅱ）二氢卟酚e（6）、铜（Ⅱ）罗丹明g（7）和铜（Ⅱ）二氢卟酚e（4）的检出限分别为3.5、1.5、3.3和1.4ng·cm ~（-3），相对标准偏差（n = 10）分别为1.8、1.6、5.2和3.6%。本文提出的反相高效液相色谱法可用于叶绿素铜钠盐组分的测定。
Copper(II) chlorophyllin, consisting of copper(II) pheophorbide a, copper(II) chlorin e(6), copper(II) rhodin g(7) and copper(II) chlorin e(4), was prepared and separated by semi-preparative high-performance liquid chromatography (HPLC). The components of copper(II) chlorophyllin were determined on a reversed-phase Inertsil ODS-2 column using a mobile phase of methanol-water (97:3, v/v) containing 1% (v/v) of acetic acid. Linear calibration plots were obtained for copper(II) chlorophyilin in the concentration range of 0-30 mu g cm(-3) with photometric detection at 407 or 423 nm. The detection limits of copper(II) pheophorbide a, copper(II) chlorin e(6), copper(II) rhodin g(7) and copper(II) chlorin e(4) were 3.5, 1.5, 3.3 and 1.4 ng cm(-3) with relative standard deviations (n = 10) of 1.8, 1.6, 5.2 and 3.6%, respectively. The reversed-phase HPLC method proposed here was demonstrated to be useful for the determination of the components of sodium copper(II) chlorophyllin.