Optical coherence tomography biospeckle imaging for fast monitoring varying surface responses of a plant leaf under ozone stress
Optical coherence tomography biospeckle imaging for fast monitoring varying surface responses of a plant leaf under ozone stress
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光学相干断层扫描生物斑点成像，用于快速监测臭氧胁迫下植物叶子的不同表面响应
DOI：
10.1117/12.2031062
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
and U. M. Rajagopalan
中科院分区：
文献类型：
--
作者：
L. K. T. Srimal;H. Kadono;and U. M. Rajagopalan
本研究利用光学相干断层扫描（OCT）研究了短期臭氧暴露对allium tuberosumplant叶片前后表面的影响。对植物叶片进行240ppb水平的臭氧处理，并从叶片的前后表面获取成像数据。利用生物斑监测生物活性的变化。计算每个点OCT时间信号的标准差（SD），并用于可视化臭氧暴露的影响。叶片背面生物散斑OCT信号SDs高于叶片正面。这些结果证明，生物散斑OCT成像技术可以对植物的短期臭氧胁迫进行连续研究。
In this study, Optical Coherence Tomography (OCT) was used to investigate the effect of short term ozone exposure on both front and back surfaces of the leaves ofAllium tuberosumplant. Plant leaves were subjected to treat with 240ppb level of ozone, and imaging data were acquired from back and front surfaces of the leaves. Variations of the biological activations were monitored based on the biospeckles. Standard deviations (SD) of OCT temporal signal at each point were calculated and used to visualize the effect of ozone exposure. Leaf back surface showed higher SDs in biospeckle OCT signal in comparison to these of the front surface. These observations prove that the short term ozone stress on plant can be investigated successively with biospeckle OCT imaging technique.