Amiloride potentiation of differentiation of human promyelocytic cell line HL-60.
Amiloride potentiation of differentiation of human promyelocytic cell line HL-60.
复制标题
阿米洛利增强人早幼粒细胞系 HL-60 的分化。
DOI：
10.1093/jnci/72.1.13
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Koeffler,HP
中科院分区：
文献类型：
--
作者：
Carlson,J;Dorey,F;CragoeJr,E;Koeffler,HP
人急性早幼粒细胞HL-60细胞暴露于二甲亚砜（DMSO）时发生分化；在这个报告中，amiloride，一种被动细胞内Na+通量的抑制剂，增强了dmso诱导的HL-60细胞的分化。在几种浓度的二甲基亚砜中可以观察到这种效应。单独使用阿米洛利不影响HL-60分化。我们测试了各种阿米洛利类似物对HL-60细胞分化的促进作用。膜溶剂（DMSO）和Na+转运抑制剂（amiloride）协同诱导髓细胞分化表明膜阳离子通量在启动分化中起重要作用。
Cells of the human acute promyelocytic cell line HL-60 undergo differentiation when exposed to dimethyl sulfoxide (DMSO); in this report, amiloride, an inhibitor of passive intracellular Na+flux, potentiated the DMSO-induced differentiation of HL-60 cells. This effect was seen at several concentrations of DMSO. Amiloride alone did not affect HL-60 differentiation. Various analogues of amiloride were tested for their ability to potentiate differentiation of HL-60 cells. The synergistic induction of myeloid differentiation by a membrane solvent (DMSO) and an Na+transport inhibitor (amiloride) suggested membrane cation flux as being important in initiating differentiation.