Transcriptome analysis of rin mutant fruit and in silico analysis of promoters of differentially regulated genes provides insight into LeMADS-RIN-regulated ethylene-dependent as well as ethylene-independent aspects of ripening in tomato
Transcriptome analysis of rin mutant fruit and in silico analysis of promoters of differentially regulated genes provides insight into LeMADS-RIN-regulated ethylene-dependent as well as ethylene-independent aspects of ripening in tomato
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DOI：
10.1007/s00438-011-0671-7
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发表时间：
2012-03-01
影响因子：
3.1
通讯作者：
Sharma, Arun K.
中科院分区：
文献类型：
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作者：
Kumar, Rahul;Sharma, Manoj K.;Sharma, Arun K.
彻底了解成熟的分子机制是对水果进行遗传操作以获得更好的货架期和营养质量的先决条件。MADS-box基因LeMADS-RIN突变导致番茄RIN果实不成熟表型。成熟抑制因子(Rin)突变体的鉴定揭示了乙烯在跃变型果实成熟调控中的重要作用。对这种突变的完全了解将揭开与果实成熟有关的新的基因调控机制。在这项研究中，包括栽培的印度番茄品种西红柿cv.PUSA Ruby和一个含有RIN突变的纯合子系(LA1795)进行了比较，以更好地了解成熟过程中RIN调节的乙烯依赖和非乙烯依赖事件。对成熟相关基因的聚类分析表明，它们在Rin突变果实中的表达谱发生了重大变化。共有112个基因在Rin突变体中表达下调，其表达模式与LeMADS-RIN在野生型果实中的表达模式相似。对这些基因存在Carg box的可能启动子以及ERE和乙烯诱导性的电子分析表明，在其调控区缺乏Carg box的基因可能受到LeMADS-RIN的间接调控。还发现了依赖乙烯的成熟方面的新调节因子。在这项研究中，我们试图区分乙烯依赖和乙烯非依赖的成熟方面，这将有助于开发策略，以改善番茄和其他作物的果实相关农艺性状。
A thorough understanding of molecular mechanisms underlying ripening is the prerequisite for genetic manipulation of fruits for better shelf-life and nutritional quality. Mutation in LeMADS-RIN, a MADS-box gene, leads to non-ripening phenotype of rin fruits in tomato. Characterization of ripening-inhibitor (rin) mutant has elucidated important role of ethylene in the regulation of climacteric fruit ripening. A complete understanding of this mutation will unravel novel genetic regulatory mechanisms involved in fruit ripening. In this study, fruit transcriptomes of two genotypes, including a cultivated Indian cultivar Solanum lycopersicum cv. Pusa Ruby and a homozygous line harboring the rin mutation (LA1795) were compared to get better insight into RIN-regulated ethylene-dependent and ethylene-independent events during ripening. Cluster analysis of ripening-related genes indicated a major shift in their expression profiles in rin mutant fruit. A total of 112 genes, exhibiting expression patterns similar to that of LeMADS-RIN in wild-type fruits, showed down regulation of expression in the rin mutant. In silico analysis of putative promoters of these genes for the presence of CArG box along with ERE and ethylene inducibility of these genes revealed that genes lacking CArG box in their regulatory regions could be indirectly regulated by LeMADS-RIN. New regulators of ethylene-dependent aspect of ripening were also identified. In this study, we have made an attempt to distinguish between ethylene-dependent and ethylene-independent aspects of ripening, which will be useful for developing strategies to improve fruit-related agronomic traits in tomato and other crops.