THE CHLOROPLAST GENOME EXISTS IN MULTIMERIC FORMS
THE CHLOROPLAST GENOME EXISTS IN MULTIMERIC FORMS
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DOI：
10.1073/pnas.86.11.4156
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发表时间：
1989-06-01
影响因子：
11.1
通讯作者：
GRUISSEM, W
中科院分区：
文献类型：
--
作者：
DENG, XW;WING, RA;GRUISSEM, W
叶绿体DNA的构象进行了分析脉冲场凝胶电泳。我们发现菠菜叶绿体DNA分子至少以四种不同的形式存在，其表观分子量分别为单体、二聚体、三聚体和四聚体。二维凝胶分析的DNA后，紫外线切口和溴化乙锭的存在下，表明它们不是异构体，不同的超螺旋密度。DNA旋转酶解链分析表明，大部分的DNA分子是低聚物，而不是索烃。通过分子杂交定量的单体、二聚体、三聚体和四聚体形式的相对量分别为1、1/3、1/9和1/27，并且在叶成熟期间不改变。对叶绿体DNA寡聚体形成的可能机制进行了讨论。
Chloroplast DNA conformation was analyzed by pulse-field gel electrophoresis. We found that spinach leaf chloroplast DNA molecules exist in at least four distinct forms with the apparent molecular weights of monomer, dimer, trimer, and tetramer. Two-dimensional gel analysis of DNA after UV nicking and in the presence of ethidium bromide indicates that they are not isomers that differ in superhelical density. DNA gyrase decatenation analysis demonstrates that the majority of the DNA molecules are oligomers rather than catenanes. The relative amounts of monomer, dimer, trimer, and tetramer forms, quantitated by molecular hybridization, are 1, 1/3, 1/9, and 1/27, respectively, and do not change during leaf maturation. The possible mechanisms of chloroplast DNA oligomer formation are discussed.