Involvement of a universal amino acid synthesis impediment in cytoplasmic male sterility in pepper.
Involvement of a universal amino acid synthesis impediment in cytoplasmic male sterility in pepper.
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DOI：
10.1038/srep23357
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发表时间：
2016-03-18
影响因子：
4.6
通讯作者：
Chai WG
中科院分区：
文献类型：
--
作者：
Fang X;Fu HF;Gong ZH;Chai WG
探讨辣椒（Capsicum annuum L.）细胞质雄性不育（CMS），研究了不育和可育辣椒花药的不同成熟过程。对不育花药的石蜡切片分析表明，绒毡层异常，细胞过度液泡化。定量蛋白质组学结果显示，在氨基酸合成途径中的组氨醇脱氢酶（HDH）、二羟酸脱氢酶（DAD）、天冬氨酸转氨酶（ATAAT）、半胱氨酸合成酶（CS）、δ-1-吡咯啉-5-羧酸合成酶（P5 CS）和谷氨酸合成酶（GS）的表达降低了1.5倍以上。此外，在保持系花药中，DAD、ATAAT、CS和P5 CS的mRNA和蛋白表达水平显示出2至16倍的增加。在花药绒毡层发育过程中，不育系的大多数氨基酸含量都有不同程度的下降，而保持系的氨基酸含量则有不同程度的上升。本研究结果表明，辣椒花药发育过程中，绒毡层发育异常伴随着氨基酸合成的显著变化，这很可能是导致辣椒雄性不育的重要原因。
To explore the mechanisms of pepper (Capsicum annuum L.) cytoplasmic male sterility (CMS), we studied the different maturation processes of sterile and fertile pepper anthers. A paraffin section analysis of the sterile anthers indicated an abnormality of the tapetal layer and an over-vacuolization of the cells. The quantitative proteomics results showed that the expression of histidinol dehydrogenase (HDH), dihydroxy-acid dehydratase (DAD), aspartate aminotransferase (ATAAT), cysteine synthase (CS), delta-1-pyrroline-5-carboxylate synthase (P5CS), and glutamate synthetase (GS) in the amino acid synthesis pathway decreased by more than 1.5-fold. Furthermore, the mRNA and protein expression levels of DAD, ATAAT, CS and P5CS showed a 2- to 16-fold increase in the maintainer line anthers. We also found that most of the amino acid content levels decreased to varying degrees during the anther tapetum period of the sterile line, whereas these levels increased in the maintainer line. The results of our study indicate that during pepper anther development, changes in amino acid synthesis are significant and accompany abnormal tapetum maturity, which is most likely an important cause of male sterility in pepper.