Protective effects of volatile agents against acetylcholine-induced bronchoconstriction in isolated perfused rat lungs
Protective effects of volatile agents against acetylcholine-induced bronchoconstriction in isolated perfused rat lungs
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DOI：
10.1111/j.1399-6576.2006.01133.x
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发表时间：
2006-10-01
影响因子：
2.1
通讯作者：
Habre, W.
中科院分区：
文献类型：
--
作者：
Lele, E.;Petak, F.;Habre, W.
背景：挥发性药物抗肺收缩的支气管活性已经得到了很好的证实。本研究的目的是研究氟烷（Hal）、异氟烷（Iso）、七氟烷（Sev）和地氟烷（Des）在没有气道张力和乙酰胆碱（Ach）诱导的支气管收缩期间改变肺力学的能力。方法：在基线条件下和向肺动脉注射Ach （0.1 mg/kg）后，收集离体、正常灌注的大鼠肺低频肺阻抗数据（Z(L)）。在实验的第一阶段，在不使用任何麻醉剂的情况下，在不使用任何挥发性药物的情况下（对照组，n = 6），或在吸入Hal （n = 6）、Iso （n = 9）、Sev （n = 6）或Des （n = 8）的最低肺泡浓度（MAC）时进行测量。气道阻力（R-aw）和实质阻尼和弹性由Z(L)数据通过模型拟合估计。结果：在基线条件下，给药Des（- 31.2 +/- 3.8%）和Sev（- 18.0 +/- 4.5%）显著降低了R-aw的基本值，而Hal和Iso对R-aw的影响没有统计学意义（分别为- 3.3 +/- 5.1%和- 8.6 +/- 2.4%）。此外，所有四种吸入麻醉剂均阻止了Ach后R-aw的增加，Hal的结果为- 14.3 +/- 11.4%，Sev的结果为- 37.5 +/- 10.9%。结论：我们在去神经离体灌注肺模型上的结果表明，Des和Sev有降低气道基础张力的潜力，而Iso和Hal在这方面无效。所有这些挥发性药物都能显著防止疼痛引起的支气管收缩。
Background: Bronchoactive properties of volatile agents against lung constriction are well established. The purpose of this study was to investigate the ability of halothane (Hal), isoflurane (Iso), sevoflurane (Sev) and desflurane (Des) to alter the lung mechanics in the absence of an airway tone and during acetylcholine (Ach)-induced bronchoconstriction.Methods: Low-frequency pulmonary impedance data (Z(L)) were collected from isolated, normo-perfused rat lungs under baseline conditions and following the injection of Ach (0.1 mg/kg) into the pulmonary artery. Measurements were performed without the administration of any anaesthetic agent in the first phase of the experiments and during inhalation without any volatile agent (control group, n = 6) or during inhalation of Hal (n = 6), Iso (n = 9), Sev (n = 6) or Des (n = 8) at 1 minimum alveolar concentration (MAC). The airway resistance (R-aw) and parenchymal damping and elastance were estimated from the Z(L) data by model fitting.Results: Under baseline conditions, the basic value of R-aw was significantly decreased by Des (- 31.2 +/- 3.8%) and Sev (- 18.0 +/- 4.5%) administration, whereas Hal and Iso did not have a statistically significant effect on R-aw (- 3.3 +/- 5.1% and - 8.6 +/- 2.4%, respectively). Moreover, all four inhalation anaesthetics prevented the increase in R-aw following Ach administration, the findings ranging between - 14.3 +/- 11.4% for Hal and - 37.5 +/- 10.9% for Sev.Conclusion: Our results on a denervated isolated perfused lung model demonstrate the potential of Des and Sev to decrease the basal airway tone, whereas Iso and Hal are ineffective in this regard. All of these volatile agents markedly protect against Ach-induced bronchoconstriction.