Gel electrophoresis of individual cells to quantify hypoxic fraction in human breast cancers.
Gel electrophoresis of individual cells to quantify hypoxic fraction in human breast cancers.
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发表时间：
1993-02
期刊：
影响因子：
11.2
通讯作者：
Peggy L. Olive;Ralph E. Durand;J. L. Riche;Ivo A. Olivotto;Stewart M. Jackson
中科院分区：
文献类型：
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作者：
Peggy L. Olive;Ralph E. Durand;J. L. Riche;Ivo A. Olivotto;Stewart M. Jackson
一种新的凝胶电泳法已经被用来定量人类肿瘤中的低氧部分。在单个肿瘤细胞中测量了辐射诱导的DNA损伤，其中观察到放射生物学低氧细胞作为一个亚群，显示DNA链断裂减少3倍。对接受姑息性放射治疗的乳腺癌患者给予5~10Gy5-10Gy单次照射，照射后立即进行细针抽吸活组织检查。在8个肿瘤中有7个检测到低氧细胞。在四种肿瘤中，DNA含量与单个细胞DNA损伤的双变量分析可以区分二倍体正常细胞和非整倍体肿瘤细胞的反应。这些早期的结果表明，“彗星分析”在解决人类肿瘤中缺氧的程度和意义方面显示出相当大的前景。
A new gel electrophoresis method has been used to quantify hypoxic fraction in human tumors. Radiation-induced DNA damage was measured in individual tumor cells, where the radiobiologically hypoxic cells were observed as a subpopulation showing a 3-fold reduction in DNA strand breaks. Patients receiving palliative radiotherapy for breast cancers were given a single dose of 5-10 Gy, and a fine needle aspiration biopsy was taken immediately after irradiation. Hypoxic cells were detected in seven of eight tumors. In four tumors, bivariate analyses of DNA content versus DNA damage to individual cells allowed distinction between the response of diploid normal cells and aneuploid tumor cells. These early results indicate that "comet assay" shows considerable promise for resolving the extent and significance of hypoxia in human tumors.