Identification of novel genes with prognostic value in childhood leukemia using cDNA microarray and quantitative RT-PCR
Identification of novel genes with prognostic value in childhood leukemia using cDNA microarray and quantitative RT-PCR
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DOI：
10.1080/08880010500457780
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发表时间：
2006-03-01
影响因子：
1.7
通讯作者：
Hara, Toshiro
中科院分区：
文献类型：
--
作者：
Hattori, Hiroyoshi;Matsuzaki, Akinobu;Hara, Toshiro
本研究的目的是鉴定基因的独特表达或抑制。应用c-DNA芯片和定量逆转录聚合酶链反应（RT-PCR）对不同预后的儿童白血病进行研究。采用基因芯片技术对44例原发性白血病细胞（急性淋巴细胞白血病28例，急性髓性白血病13例，急性淋巴细胞白血病13例）中所选基因的表达水平进行了定量分析。短暂性骨髓增生性疾病，3)。CDKN2C、CRADD和IGFBP-2基因的表达水平与AML患者的无事件生存期显著相关。目前的结果表明，cDNA微阵列和定量RT-PCR的结合可能有助于鉴定儿童AML中具有预后价值的新基因。
The aim, of this study was to identify genes distinctively expressed or suppressed in. childhood leukemia with different prognoses using c-DNA microarray and quantitative reverse transcription-polymerase chain reaction, (RT-PCR). The expression levels of the selected genes by cDNA microarray were quantified in primary leukemic blasts from 44 patients (acute lymphoblastic leukemia, 28; acute myelogenous leukemia (AML), 13; transient myeloproliferative disorder, 3). The expression levels of CDKN2C, CRADD, and IGFBP-2 genes were significantly associated with the event-free survival of the patients in AML. The present results suggest that a combination of cDNA microarroy and quantitative RT-PCR may be useful to identify novel genes with prognostic value in childhood AML.