Cdx2 protein expression in normal and malignant human tissues: An immunohistochemical survey using tissue microarrays
Cdx2 protein expression in normal and malignant human tissues: An immunohistochemical survey using tissue microarrays
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DOI：
10.1097/01.mp.0000086073.92773.55
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发表时间：
2003-09-01
期刊：
影响因子：
7.5
通讯作者：
Frierson, HF
中科院分区：
文献类型：
--
作者：
Moskaluk, CA;Zhang, H;Frierson, HF
Cdx 2已在RNA增殖实验中被鉴定为结肠癌的标志物。我们在这里表明，Cdx 2蛋白的免疫组化检测相关以及与RNA转录水平的寡核苷酸微阵列检测。使用含有大多数正常组织类型和Cdx 2蛋白抗体的组织微阵列，仅在小肠和大肠上皮细胞核和部分胰管系统中观察到强弥漫性Cdx 2染色。在包含来自许多解剖部位的745个癌症的组织微阵列中，结肠腺癌在90%的病例中显示出强烈的广泛染色，而胃、食管和卵巢的腺癌（粘液样和粘液型）仅在20-30%的病例中显示出广泛染色。其他类型的癌仅在小于或等于1%的病例中显示出广泛的染色。在30例神经内分泌肿瘤中，中肠和后肠类癌最多（分别为73%和44%），因此与腺癌中Cdx 2表达的分布平行。Cdx 2在人类组织和肿瘤形成的谱中显示出有限的表达范围，因此在某些临床情况下可用于确定肿瘤的起源部位。
Cdx2 has been identified as a marker of colon cancer in RNA-prolifing experiments. We show here that the detection of Cdx2 protein by immunohistochemistry correlates well with RNA transcript levels as detected by oligonucleotide microarrays. Using tissue microarrays containing most normal tissue types and an antibody to the Cdx2 protein, strong diffuse Cdx2 staining was only seen in the nuclei of small and large intestinal epithelium and portions of the pancreatic duct system. In tissue microarrays containing 745 cancers from many anatomic sites, colonic adenocarcinomas showed strong extensive staining in 90% of cases, with adenocarcinomas of the stomach, esophagus, and ovary (endometrioid and mucinous types) showing extensive staining in only 20-30% of cases. Other types of carcinomas showed extensive staining in only less than or equal to1% of cases. Of 30 neuroendocrine tumors examined, carcinoids of the midgut and hindgut had the most cases with extensive staining (73% and 44%, respectively), thus paralleling the distribution of Cdx2 expression in adenocarcinomas. Cdx2 shows a limited range of expression in the spectrum of human tissues and neoplasia and thus may have utility in determining the site of origin of tumors in certain clinical situations.