Visualizing Notch signaling in vivo in Drosophila tissues.
Visualizing Notch signaling in vivo in Drosophila tissues.
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DOI：
10.1007/978-1-4939-1139-4_8
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发表时间：
2014
影响因子：
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通讯作者：
B. Housden;Jinghua Li;S. Bray
中科院分区：
文献类型：
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作者：
B. Housden;Jinghua Li;S. Bray
体内Notch通路活性可视化对于研究Notch信号传导的功能和机制非常有价值。已经开发了多种工具来监测果蝇中的通路活性，包括内源性 Notch 响应基因和合成转录报告基因构建体。在这里，我们总结了一些可用的不同 Notch 信号报告基因，讨论了它们的相对优点，并描述了两种可视化其表达的方法（免疫染色和 X-gal 染色）。这些方法广泛适用于果蝇发育的一系列组织和阶段。
The ability to visualize Notch pathway activity in vivo is invaluable for studying the functions and mechanisms of Notch signaling. A variety of tools have been developed to enable monitoring of pathway activity inDrosophila, including endogenous Notch-responsive genes and synthetic transcriptional reporter constructs. Here we summarize some of the different Notch signaling reporters that are available, discuss their relative merits, and describe two methods for visualizing their expression (immunostaining and X-gal staining). These approaches are widely applicable to a range of tissues and stages inDrosophiladevelopment.