Profiling TRA-1-81 antigen distribution on a human embryonic stem cell.
Profiling TRA-1-81 antigen distribution on a human embryonic stem cell.
复制标题
分析人类胚胎干细胞上 TRA-1-81 抗原的分布。
DOI：
10.1016/j.bbrc.2008.02.102
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发表时间：
2008
影响因子：
3.1
通讯作者：
Wang,Rong
中科院分区：
文献类型：
--
作者：
Qiu,Dengli;Xiang,Jialing;Li,Zhaoxia;Krishnamoorthy,Aparna;Chen,Liaohai;Wang,Rong
人类胚胎干细胞（hES）在再生医学中具有巨大的前景。虽然hES细胞具有无限的自我更新潜力，但它们倾向于在培养中自发分化。TRA-1-81是未分化hES细胞的生物标志物。单细胞中TRA-1-81表达水平的定量表征有助于捕获“开启”信号并理解早期分化的机制。在这里，我们报告我们的检查TRA-1-81的分布和协会的hES细胞膜上使用原子力显微镜（AFM）。我们的研究结果表明，TRA-1-81抗原的聚集分布是未分化的hES细胞的特征。我们还分别在未分化细胞和自发分化细胞上评估了每细胞约17，800个表位和约700个表位的TRA-1-81表达水平。本研究中的方法可以适用于检查各种细胞类型上的其他表面蛋白，从而为在单细胞水平上研究蛋白质分布和缔合提供了通用工具。
Human embryonic stem (hES) cells hold great promise in regenerative medicine. Although hES cells have unlimited self-renewal potential, they tend to differentiate spontaneously in culture. TRA-1-81 is a biomarker of undifferentiated hES cells. Quantitative characterization of TRA-1-81 expression level in a single cell helps capture the “turn-on” signal and understand the mechanism of early differentiation. Here, we report on our examination of TRA-1-81 distribution and association on a hES cell membrane using an atomic force microscope (AFM). Our results suggest that aggregated distribution of TRA-1-81 antigen is characteristic for undifferentiated hES cells. We also evaluated the TRA-1-81 expression level at ∼17,800 epitopes and ∼700 epitopes per cell on an undifferentiated cell and a spontaneously differentiated cell, respectively. The method in this study can be adapted in examining other surface proteins on various cell types, thus providing a general tool for investigating protein distribution and association at the single cell level.