Stem cell properties of human periodontal ligament cells
Stem cell properties of human periodontal ligament cells
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DOI：
10.1111/j.1600-0765.2006.00870.x
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发表时间：
2006-08-01
影响因子：
3.5
通讯作者：
Ishikawa, I
中科院分区：
文献类型：
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作者：
Nagatomo, K;Komaki, M;Ishikawa, I
背景和目的：干细胞已被用于各种领域的再生治疗。人牙周膜(PDL)细胞中具有干细胞特性的细胞比例尚不清楚。材料和方法：从提取的前磨牙或智齿中获得PDL细胞，然后进行自我更新的增殖实验、多向分化实验、Stro-1的免疫染色和干细胞标志物(包括CD105、CD166和Stro-1)的FACS分析。结果：在400个PDL细胞中，约30%的细胞具有复制潜能并形成单细胞集落，其中30%的细胞Stro-1染色阳性20%分化为脂肪细胞，30%分化为成骨细胞。流式细胞仪分析显示PDL细胞，包括细胞群，表达干细胞标志物CD105、CD166和Stro-1。结论：PDL细胞具有重要的干细胞特性，如自我更新和多潜能，并在其细胞表面表达间充质干细胞标志物CD105、CD166和Stro-1，但存在一定的变异。因此，PDL细胞可用于牙周再生手术。
Background and Objective: Stem cells have been used for regenerative therapies in various fields. The proportion of cells that possess stem cell properties in human periodontal ligament (PDL) cells is not yet well understood. In this study, we quantitatively characterized human PDL cells to clarify their stem cell properties, including self-renewal, multipotency, and stem cell marker expression.Material and Methods: PDL cells were obtained from extracted premolar or wisdom teeth, following which a proliferation assay for self-renewal, a differentiation assay for multipotency, immunostaining for STRO-1, and fluorescence-activated cell sorter (FACS) analysis for stem cell markers (including CD105, CD166, and STRO-1) were performed.Results: Approximately 30% of 400 PDL cells were found to possess replicative potential and formed single-cell colonies, and 30% of these colonies displayed positive staining for STRO-1, 20% differentiated into adipocytes and 30% differentiated into osteoblasts. FACS analysis revealed that PDL cells, including cell populations, expressed the stem cell markers CD105, CD166, and STRO-1.Conclusion: The findings of this study indicated that PDL cells possess crucial stem cell properties, such as self-renewal and multipotency, and express the mesenchymal stem cell markers CD105, CD166, and STRO-1 on their cell surface, although there were some variations. Thus, PDL cells can be used for periodontal regenerative procedures.