Interaction of amphotericin B with monolayers of egg lecithin and cholesterol: polorized absorption spectra
Interaction of amphotericin B with monolayers of egg lecithin and cholesterol: polorized absorption spectra
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两性霉素 B 与单层鸡蛋卵磷脂和胆固醇的相互作用：偏振吸收光谱
DOI：
10.1016/0005-2736(74)90189-8
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
N. Ockman
中科院分区：
文献类型：
--
作者：
N. Ockman
在低表面压和高表面压条件下，获得了抗生素多烯类抗生素阿替霉素B与卵磷脂、胆固醇及等摩尔卵磷脂-胆固醇单分子膜相互作用的偏振吸收光谱。推导出一个表达式，使多烯吸收带在4077和3645 Ω的过渡时刻的取向的偏振数据的确定。对于某些系统，3645- 1000频段被以前未报告的出现在3610和3632之间的频段所取代。发现4077-π跃迁矩的取向（平行于分子长轴）从胆固醇低压单层的64 °到卵磷脂-胆固醇高压膜的21 °变化。对于3610 °和3645 °之间的带，角度从胆固醇的90 °到高压混合脂质膜的18 °不等。结果发现，胆固醇和卵磷脂-胆固醇单分子膜的表面压力的大幅度增加导致两种膜的4077-磁矩的角度减小，并且混合膜的更高能量的磁矩的角度减小。增加胆固醇在这些单分子膜中的含量旋转的过渡时刻的取向，为两个带朝向表面法线，变化是最大的低压膜。阿替霉素B降低这些脂质单层表面张力的有效性与其结合、定向和渗透程度有关。对于低压胆固醇膜，其中与多烯的表面相互作用是最大的，结合和渗透是大的，并且多烯分子取向为其长尺寸几乎垂直于表面。
Polarized absorption spectra have been obtained of the antibiotic polyene, amphotericin B, interacting with monolayers of egg lecithin, cholesterol and equimolar egg lecithin-cholesterol at low and high surface pressures. An expression is derived which enables the determination from the polarization data of the orientations of the transition moments of the polyene absorption bands at 4077 and 3645 Å. For some of the systems the 3645-Å band is replaced by a previously unreported band appearing between 3610 and 3632 Å. The orientation of the 4077-Å transition moment (parallel to the long molecular axis) is found to vary from an angle of 64 ° with the surface for the low-pressure monolayers of cholesterol to 21 ° for the high-pressure films of egg lecithin-cholesterol. For the band between 3610 Å and 3645 Å, the angle varies from 90 ° for cholesterol to 18 ° for the high-pressure mixed-lipid film. It is found that a large increase in surface pressure of the cholesterol and egg lecithin-cholesterol monolayers causes a decrease in the angle of the 4077-Å moment for both films and that of the higher energy moment for the mixed film. Increasing the content of cholesterol in these monolayers rotates the orientation of the transition moments for both bands toward the surface normal, the change being greatest for the low-pressure films. The effectiveness of amphotericin B in lowering the surface tension of these lipid monolayers is related to its binding, orientation and extent of penetration. For low-pressure cholesterol films where the surface interaction with the polyene is greatest, the binding and penetration are large and the polyene molecule is oriented with its long dimension nearly perpendicular to the surface.