Retinol Status and Expression of Retinol-Related Proteins in Methionine-Choline Deficient Rats
Retinol Status and Expression of Retinol-Related Proteins in Methionine-Choline Deficient Rats
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DOI：
10.3177/jnsv.60.78
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发表时间：
2014-04-01
影响因子：
1.6
通讯作者：
Tamai, Hiroshi
中科院分区：
文献类型：
--
作者：
Miyazaki, Hiroshi;Takitani, Kimitaka;Tamai, Hiroshi
视黄醇及其衍生物维甲酸具有多种功能，包括视觉、免疫、造血、生殖、细胞分化/生长和发育。非酒精性脂肪性肝病(NAFLD)是发达国家最常见的疾病之一，包括从脂肪变性到脂肪性肝炎的广泛形式，后者进一步发展为肝硬变。视黄醇状态在肝脏动态平衡中起着重要作用。本研究旨在探讨蛋氨酸-胆碱缺乏(MCD)大鼠作为NAFLD模型的视黄醇状态及视黄醇相关蛋白，包括酶和结合蛋白的表达。我们检测了血浆、肝脏和基因中的视黄醇水平。β-胡萝卜素15，15‘-单加氧酶(BCMO)、卵磷脂：视黄醇酰基转移酶(LRAT)、乙醛脱氢酶1A1(ALDH1A1)、ALDH1A2和细胞视黄醇结合蛋白(CRBP)-I在MCD大鼠中的表达。MCD大鼠血浆视黄醇水平低于对照组，肝视黄醇水平高于对照组。MCD大鼠肠、肝组织中BCMO的表达低于对照组，而睾丸和肾脏中BCMO的表达高于对照组。LRAT、CRBP-I、ALDH1A1和ALDH1A2在MCD大鼠肝脏中的表达也较高。视黄醇相关蛋白表达的改变可能影响NAFLD的视黄醇状态。
Retinol and its derivative, retinoic acid, have pleiotropic functions including vision, immunity, hematopoiesis, reproduction, cell differentiation/growth, and development. Non-alcoholic fatty liver disease (NAFLD) is one of the most common diseases in developed countries and encompasses a broad spectrum of forms, ranging from steatosis to steatohepatitis, which develops further to cirrhosis. Retinol status has an important role in liver homeostasis. The purpose of this study was to evaluate the retinol status and expression of retinol-related proteins, including enzymes and binding proteins, in methionine-choline deficient (MCD) rats as a model of NAFLD. We examined retinol levels in the plasma and liver and gene. expression for beta-carotene 15,15'-monooxygenase (BCMO), lecithin:retinol acyltransferase (LRAT), aldehyde dehydrogenase 1A1 (ALDH1A1), ALDH1A2, and cellular retinol binding protein (CRBP)-I in MCD rats. The plasma retinol levels in MCD rats were lower than those in the controls, whereas hepatic retinol levels in MCD rats were higher. BCMO expression in the intestine and liver in MCD rats was lower, whereas that in the testes and the kidneys was higher than in control rats. Expression of LRAT, CRBP-I, ALDH1A1, and ALDH1A2 in the liver of MCD rats was also higher. Altered expression of retinol-related proteins may affect retinol status in NAFLD.