A comparison of blood-brain barrier and blood-nerve barrier endothelial cell markers
A comparison of blood-brain barrier and blood-nerve barrier endothelial cell markers
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DOI：
10.1007/s004290050248
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发表时间：
1999-06-01
期刊：
影响因子：
--
通讯作者：
Allt, G
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文献类型：
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作者：
Orte, C;Lawrenson, JG;Allt, G
血脑屏障(BBB)处的内皮细胞(EC)的许多主要特性已被证明是星形胶质细胞依赖的。雪旺细胞是否诱导和维持了血神经屏障(BNB)上的类似特性，目前还没有研究。作为初步调查，我们对血脑屏障、血脑屏障和神经束膜上的六种EC膜标志物进行了比较研究。用免疫印迹和免疫细胞化学方法测定了葡萄糖转运体(GLUT-1)、转铁素受体(OX-26)、内皮屏障抗原(EBA)和OX-47抗原在大鼠大脑皮质和坐骨神经之间的相对分布。用酶细胞化学方法对γ-谷氨酰转肽酶(GGTP)和碱性磷酸酶进行同样的比较。免疫细胞化学结果显示，GLUT-1在脑内皮细胞、神经内皮细胞和神经膜均有较强的表达。OX-26在脑内皮细胞中呈强阳性表达，在神经内皮细胞和神经外膜中仅有微量表达。同样，EBA在脑内皮细胞中呈强阳性表达，在神经内皮细胞中呈微量阳性，但在神经外膜中未见表达。OX-47在脑内皮细胞和神经外膜有中等表达，而在神经内皮细胞中无表达，定量免疫印迹显示EBA和OX-26在脑内皮细胞和坐骨神经匀浆中的表达水平差异有统计学意义。酶细胞化学显示GGTP在脑内皮细胞呈强阳性反应，而在神经内皮细胞和神经膜中无表达。碱性磷酸酶在脑内皮细胞和神经内皮细胞中呈强阳性表达，在神经外膜中不表达。综上所述，与脑相比，这六种膜标志物在神经中的表达是不均匀的。这种神经分布模式不能简单地用星形胶质细胞的缺失及其诱导影响来解释，雪旺细胞的任何诱导影响都需要研究。
A number of major properties of endothelial cells (EC) at the blood-brain barrier (BBB) have been shown to be astrocyte-dependent. Whether analogous properties at the blood-nerve barrier (BNB) are induced and maintained by Schwann cells has not been investigated. As a preliminary investigation we have undertaken a comparative study of six EC membrane markers at the BBB and BNB and perineurium. Employing immunoblotting and immunocytochemistry the relative distribution between rat brain cortex and sciatic nerve was determined for the glucose transporter (GLUT-1), the transferin receptor (OX-26), the endothelial barrier antigen (EBA) and the OX-47 antigen. Using enzyme cytochemistry the same comparison was made for gamma-glutamyl transpeptidase (GGTP) and alkaline phosphatase. By immunocytochemistry GLUT-1 was uniformly strongly represented in brain EC, nerve EC and perineurium. OX-26 was strongly positive in brain EC but present only in trace quantities in nerve EC and perineurium. EBA similarly showed strong positivity in brain EC and trace amounts in nerve EC but was absent from perineurium. OX-47 was present moderately in brain EC and perineurium but absent from nerve EC, Quantitative immunoblotting of brain and sciatic nerve homogenates showed statistically significant differences in the level of expression of EBA and OX-26 between the two tissues. Enzyme cytochemistry showed that GGTP was strongly positive in brain EC but absent from nerve EC and perineurium. Alkaline phosphatase stained strongly in brain and nerve EC and was absent from perineurium. In summary the six membrane markers were heterogeneously represented in nerve compared with brain. This pattern of distribution in the nerve cannot simply be accounted for by the absence of astrocytes and their inductive influences, Any inductive influences of Schwann cells require investigation.