Eukaryotic mRNAs encoding abundant and scarce proteins are statistically dissimilar in many structural features
Eukaryotic mRNAs encoding abundant and scarce proteins are statistically dissimilar in many structural features
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DOI：
10.1016/s0014-5793(98)01482-3
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发表时间：
1998-12-04
期刊：
影响因子：
3.5
通讯作者：
Kolchanov, NA
中科院分区：
文献类型：
--
作者：
Kochetov, AV;Ischenko, IV;Kolchanov, NA
众所周知，非编码mRNA序列在许多结构特征上是不相似的。对于单个mRNA，发现这些特征中的一些与它们的翻译效率相关，然而，没有进行系统的统计分析来关联编码给定蛋白质的mRNA的蛋白质丰度和结构特征。我们已经证明，真核生物中编码高丰度和低丰度蛋白质的mRNA的结构和上下文特征在5'非翻译区（UTR）上存在差异。统计学上，低表达mRNA的5' UTR较长，其鸟嘌呤和胞嘧啶含量较高，它们具有主开放阅读框的翻译起始密码子的较不理想的环境，并且含有比5'UTR更频繁的上游AUG，高表达的mRNA，除了5'UTR的差异外，高表达的mRNA含有更强的终止信号。低表达和高表达mRNA的结构特征可能有助于其蛋白质产物的产量。(C)1998年欧洲生物化学学会联合会。
It is well known that non-coding mRNA sequences are dissimilar in many structural features. For individual mRNAs correlations were found for some of these features and their translational efficiency, However, no systematic statistical analysis was undertaken to relate protein abundance and structural characteristics of mRNA encoding the given protein. We have demonstrated that structural and contextual features of eukaryotic mRNAs encoding high- and low-abundant proteins differ in the 5' untranslated regions (UTR), Statistically, 5' UTRs of low-expression mRNAs are longer, their guanine plus cytosine content is higher, they have a less optimal context of the translation initiation codons of the main open reading frames and contain more frequently upstream AUG than 5' UTRs of high-expression mRNAs, Apart from the differences in 5' UTRs, high-expression mRNAs contain stronger termination signals. Structural features of low- and high-expression mRNAs are likely to contribute to the yield of their protein products. (C) 1998 Federation of European Biochemical Societies.