Effects of negative pressure wound therapy on mesenchymal stem cells proliferation and osteogenic differentiation in a fibrin matrix.
Effects of negative pressure wound therapy on mesenchymal stem cells proliferation and osteogenic differentiation in a fibrin matrix.
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负压伤口治疗对纤维蛋白基质中间充质干细胞增殖和成骨分化的影响
DOI：
10.1371/journal.pone.0107339
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hu X
中科院分区：
文献类型：
--
作者：
Zhu J;Yu A;Qi B;Li Z;Hu X
真空辅助闭合负压创面治疗（NPWT）是治疗顽固性创面的有效方法。然而，其在骨愈合中的作用尚不清楚。在这里，我们研究了NPWT对大鼠骨膜来源的间充质干细胞（P-MSCs）增殖和成骨细胞分化在三维纤维蛋白基质中的影响。P-MSCs在构建细胞凝块之前进行三代原代培养。将纤维蛋白凝块与NPWT在亚大气灌注生物反应器中连续吸入- 125 mmHg孵育。暴露在大气压力下的凝块作为静态控制。与对照组相比，NPWT组培养3 d后细胞增殖显著增加。两组细胞凋亡率比较，差异无统计学意义。持续抽吸后，P-MSCs的ALP活性和矿化均增加。NPWT组1型胶原蛋白和转录因子Cbfa-1的表达在1、3、7天时间点较高，骨钙素和整合素β5的表达在3、7天时间点较高。这些结果表明，NPWT在−125 mmHg持续吸压下短时间处理可以增强P-MSCs的细胞增殖，并诱导其向成骨表型分化。我们发现NPWT治疗后机械转导分子整合素β5高表达，提示NPWT可能通过促进骨形成和减少骨吸收在骨折愈合中发挥积极作用。
Vacuum-assisted closure (VAC) negative pressure wound therapy (NPWT) has been proven to be an effective therapeutic method for the treatment of recalcitrant wounds. However, its role in bone healing remains to be unclear. Here, we investigated the effects of NPWT on rat periosteum-derived mesenchymal stem cells (P-MSCs) proliferation and osteoblastic differentiation in a 3D fibrin matrix. P-MSCs underwent primary culture for three passages before being used to construct cell clots. The fibrin clots were incubated with NPWT under continuous suction at −125 mmHg in a subatmospheric perfusion bioreactor. Clots exposed to atmospheric pressure served as the static control. Compared to the control group, cell proliferation significantly increased in NPWT group after incubation for 3 days. There was no statistical difference in apoptosis rate between two groups. The ALP activity and mineralization of P-MSCs all increased under continuous suction. The expressions of collagen type 1 and transcription factor Cbfa-1 were higher at the 1-, 3-, and 7-day timepoints and the expressions of osteocalcin and integrin β5 were higher at the 3-, and 7-day timepoints in the NPWT group. These results indicate that a short time treatment with NPWT, applied with continuous suction at −125 mmHg, can enhance cellular proliferation of P-MSCs and induce the differentiation toward an osteogenic phenotype. The mechanotransduction molecule integrin β5 was found to be highly expressed after NPWT treatment, which indicates that NPWT may play a positive role in fracture healing through enhance bone formation and decrease bone resorption.
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影响因子：
5
作者：
Coudert, Amelie E.;Del Fattore, Andrea;de Vernejoul, Marie-Christine
通讯作者：
de Vernejoul, Marie-Christine
三维培养成纤维细胞的微变形诱导基因表达和形态变化
影响因子：
1.5
作者：
Lu, Feng;Ogawa, Rei;Orgill, Dennis P.
通讯作者：
Orgill, Dennis P.
影响因子：
4.1
作者：
Huang, Chenyu;Ogawa, Rei
通讯作者：
Ogawa, Rei
影响因子：
3.7
作者：
Liu Y;Zhou Q;Wang Y;Liu Z;Dong M;Wang Y;Li X;Hu D
通讯作者：
Hu D
负压治疗刺激兔子中关键大小的钙钙缺损的愈合。
DOI：
10.1038/bonekey.2013.33
发表时间：
2013
期刊：
BoneKEy reports
影响因子：
--
作者：
Swain, Larry D;Cornet, Douglas A;Manwaring, Michael E;Collins, Barbara;Singh, Vinay K;Beniker, Dan;Carnes, David L
通讯作者：
Carnes, David L