Identification and characterization of Drosophila homolog of Rho-kinase
Identification and characterization of Drosophila homolog of Rho-kinase
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DOI：
10.1016/s0378-1119(99)00351-0
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发表时间：
1999-10-01
期刊：
影响因子：
3.5
通讯作者：
Nishida, Y
中科院分区：
文献类型：
--
作者：
Mizuno, T;Amano, M;Nishida, Y
Rho家族的小GTP酶及其相关的调节剂和目标是在发展中的各种形态发生事件的重要介质。哺乳动物Rho激酶/ROK α是Rho的靶点之一，已显示以GTP结合形式与Rho结合，并磷酸化肌球蛋白轻链（MLC）和肌球蛋白磷酸酶的肌球蛋白结合亚基（MBS），导致肌球蛋白的活化。因此，Rho激酶/ROK α已被认为在应力纤维和局灶性粘连的形成中发挥重要作用。我们已经鉴定了Rho激酶/ROK α的果蝇同源物DRho激酶，其具有Rho激酶/ROK α的基本结构特征，包括N-末端激酶、中央卷曲螺旋和C-末端普列克底物蛋白同源（PH）结构域。一个双杂交分析表明，DRHO-激酶相互作用与GTP结合形式的果蝇Rho，Drhol，在保守的Rho结合位点。DRho激酶在体外可磷酸化牛Rho激酶的优选底物MLC和MBS。DRho激酶在整个发育过程中普遍表达，其模式与Drhol基本相同。这些结果表明，DRho-激酶是Drhol的效应子。(C)1999 Elsevier Science B. V.保留所有权利。
The Rho family of small GTPases and their associated regulators and targets are essential mediators of diverse morphogenetic events in development. Mammalian Rho-kinase/ROK alpha, one of the targets of Rho, has been shown to bind to Rho in GTP-bound form and to phosphorylate the myosin light chain (MLC) and the myosin-binding subunit (MBS) of myosin phosphatase, resulting in the activation of myosin. Thus, Rho-kinase/ROK alpha has been suggested to play essential roles in the formation of stress fibers and focal adhesions. We have identified the Drosophila homolog of Rho-kinase/ROK alpha, DRho-kinase, which has conserved the basic structural feature of Rho-kinase/ROK alpha consisting of the N-terminal kinase, central coiled-coil and C-terminal pleckstrin homology (PH) domains. A two-hybrid analysis demonstrated that DRho-kinase interacts with the GTP-bound form of the Drosophila Rho, Drhol, at the conserved Rho-binding site. DRho-kinase can phosphorylate MLC and MBS, preferable substrates for bovine Rho-kinase, in vitro. DRho-kinase is ubiquitously expressed throughout development, in a pattern essentially identical to that of Drhol. These results suggest that DRho-kinase is an effector of Drhol. (C) 1999 Elsevier Science B.V. All rights reserved.