Postnatal changes in oxygen affinity of sheep red cells: a dual role of diphosphoglyceric acid.
Postnatal changes in oxygen affinity of sheep red cells: a dual role of diphosphoglyceric acid.
复制标题
绵羊红细胞的产后氧亲和力变化：二磷酸甘油酸的双重作用。
DOI：
10.1152/ajplegacy.1970.219.1.217
复制
发表时间：
1970
期刊：
影响因子：
--
通讯作者：
G. Meschia
中科院分区：
文献类型：
--
作者：
F. Battaglia;H. McGaughey;E. Makowski;G. Meschia
方法本研究使用的绵羊均为纯种多赛特羊。胎儿血液是在妊娠最后三分之一时从脐静脉采集的。成年绵羊血液取自未怀孕的母羊。在宫外生命的前 2 个月内获取 Oz 解离曲线和红细胞有机磷酸盐浓度的血样。氧解离曲线。氧解离曲线的构建如前所述 (7)。 pH 和 Paz 在 39.6°C 下测量。POT 使用连接到 Beckman 气体分析仪（型号 160）的 Clark 型电极进行测量。在每个 PO 之前和之后，使用与血样范围相同的 Pea 对标准气体混合物进行氧张力测定：！对血液样本的测量。 pH 值使用 Radiometer 4 型 pH 计和 E 5020 型微电极链进行测量。在胎儿和成人血液中，使用辐射计血氧计对溶血的浓缩红细胞进行分光光度法测量氧饱和度。每天通过使用 Beckman GC-2A 气相色谱仪测定选定血液样本的氧含量和容量来校准血氧计。由于产后从羔羊获得的血液可能含有在氧亲和力方面更加异质的红细胞群体，因此这些血液样品的O 2 饱和度是通过气相色谱法测定氧含量和容量来计算的，以避免使用分光光度技术引入的任何误差。氧解离曲线的结果以一般方程的形式表示：
METHODSThe sheep used in this study were all purebred Dorsets. Fetal blood was obtained from the umbilical vein in the last third of pregnancy. Adult sheep blood was obtained from nonpregnant ewes. Blood samples for Oz-dissociation curves and red cell organic phosphate concentrations were obtained in the first 2 months of extrauterine life. Oxygen-dissociation curves. The oxygen-dissociation curves were constructed as described previously (7). pH and Paz were measured at 39.6 C. The POT was measured with a Clark-type electrode connected to a Beckman gas analyzer, model 160. Oxygen tension determinations on a standard gas mixture, with a Pea in the same range as the blood samples, were run before and after each PO:! measurement on a blood sample. pH was measured with a Radiometer model 4 pH meter and a type E 5020 microelectrode chain. In fetal and adult blood, oxygen saturations were measured spectrophotometrically with a Radiometer oximeter on hemolyzed packed red cells. The oximeter was calibrated each day by determining oxygen contents and capacities on selected blood samples with a Beckman GC-2A gas chromatograph. Since blood obtained from lambs postnatally may contain a more heterogeneous population of red cells in terms of their oxygen affinities, the 02 saturations of these blood samples were calculated from the determination of oxygen contents and capacities by gas chromatography to avoid any errors introduced by the use of spectrophotometric techniques. The results of the oxygen-dissociation curve were expressed in the form of a general equation: