A deletion in the PHYD gene of the Arabidopsis Wassilewskija ecotype defines a role for phytochrome D in red/far-red light sensing
A deletion in the PHYD gene of the Arabidopsis Wassilewskija ecotype defines a role for phytochrome D in red/far-red light sensing
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DOI：
10.1105/tpc.9.8.1317
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发表时间：
1997-08-01
期刊：
影响因子：
11.6
通讯作者：
Sharrock, RA
中科院分区：
文献类型：
--
作者：
Aukerman, MJ;Hirschfeld, M;Sharrock, RA
拟南芥的Wassilewskija（Ws）生态型的PHYD基因包含从阅读框的氨基酸29开始的14-bp缺失（phyD-1突变），导致在缺失终点下游138个核苷酸处的无义密码子处的翻译终止。免疫印迹分析显示Ws缺乏phyD，但含有正常水平的phyA、phyB和phyC。通过回交到Ws和兰茨贝格直立遗传背景中，我们构建了PHYD+和phyD-1系以及phyB（-）PHYD+和phyB（-）phyD（-）系的同胞对。在白色或红光下生长的下胚轴长度在B+D+、B+D-、B-D+和B-D-菌株中依次增加。在Ws遗传背景下，叶柄长度增加，子叶面积减少，幼苗茎中花色素苷积累减少，日终脉冲远红光对下胚轴伸长的影响减弱，开花开始时莲座叶数量减少。因此，在氨基酸序列上与phyB相似80%的phyD，与phyB共同调节许多避荫反应。显然自然发生的phyD-1突变的存在表明phyD在某些自然环境中不是必需的。
The PHYD gene of the Wassilewskija (Ws) ecotype of Arabidopsis contains a 14-bp deletion (the phyD-1 mutation) beginning at amino acid 29 of the reading frame, resulting in translation termination at a nonsense codon 138 nucleotides downstream of the deletion end point, Immunoblot analyses showed that Ws lacks phyD but contains normal levels of phyA, phyB, and phyC. By backcrossing into the Ws and Landsberg erecta genetic backgrounds, we constructed sibling pairs of PHYD+ and phyD-1 lines and of phyB(-) PHYD+ and phyB(-) phyD(-) lines. Hypocotyl lengths after growth under white or red light increased sequentially in strains that were B+D+, B+D-, B-D+, and B-D-. In the Ws genetic background, an increase in petiole length, a reduction in cotyledon area and in anthocyanin accumulation in seedling stems, a diminished effect of an end-of-day pulse of far-red light on hypocotyl elongation, and a decrease in the number of rosette leaves at the onset of flowering were also seen sequentially in these lines, Thus, phyD, which is similar to 80% identical in amino acid sequence to phyB, acts in conjunction with phyB in regulating many shade avoidance responses. The existence of the apparently naturally occurring phyD-1 mutation indicates that phyD is not essential in some natural environments.