Enantioselective effect of chiral fungicide prothioconazole on Fusarium graminearum: Fungicidal activity and DON biosynthesis
Enantioselective effect of chiral fungicide prothioconazole on Fusarium graminearum: Fungicidal activity and DON biosynthesis
复制标题
手性杀菌剂丙硫菌唑对禾谷镰刀菌的对映选择性作用：杀菌活性和 DON 生物合成
DOI：
10.1016/j.envpol.2022.119553
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Chenglan Liu
中科院分区：
文献类型：
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作者：
Chaofeng Li;Chenglan Liu
丙硫菌唑是一种手性三唑类杀菌剂，广泛用于防治小麦赤霉病。禾谷镰刀菌（F.禾谷镰刀菌（graminearum）是FHB的主要病原菌，能产生包括脱氧雪腐镰刀菌烯醇（deoxynivalenol，DON）在内的多种次生代谢产物，严重威胁着人类和动物的健康。然而，一些杀菌剂可能会刺激F。在一定条件下，禾谷镰刀菌DON的合成量最大。迄今为止，丙硫菌唑对映体的杀菌活性和对映体选择性对真菌DON产生、转录组和代谢组的影响尚不清楚。graminearum不清楚。研究了R-（-）-丙硫菌唑对F.在所有条件下，禾谷镰刀菌的抑菌活性都是S-（+）-丙硫菌唑的9.12-17.73倍。丙硫菌唑对映体能诱导F.在水分活度（aw）为0.99、温度为30 °C时，禾谷镰刀菌DON合成量最大，尤其是R-（-）-丙硫菌唑。R-（-）-丙硫菌唑处理的TRI 6、TRI 10和TRI 101的表达水平显著高于S-（+）-丙硫菌唑处理。糖酵解、丙酮酸代谢、雷帕霉素靶蛋白（TOR）信号转导途径和环磷酸腺苷（cAMP）-蛋白激酶A（PKA）信号转导途径中的大多数基因在R-（-）-丙硫菌唑处理下的表达水平高于S-（+）-丙硫菌唑处理和对照。与R-（-）-丙硫菌唑和对照相比，S-（+）-丙硫菌唑处理下过氧化物酶体途径显示出更高的转录活性。代谢组学研究表明，R-（-）-丙硫菌唑对苯丙氨酸代谢有显著影响，S-（+）-丙硫菌唑对苯丙氨酸代谢无明显富集作用。这些结果有助于了解丙硫菌唑对映体对F.并揭示了丙硫菌唑对映体的相关潜在机制。
Prothioconazole, a chiral triazole fungicide, is widely used to control Fusarium head blight (FHB) of wheat. Fusarium graminearum (F. graminearum), as the main pathogen of FHB, can produce many secondary metabolites including deoxynivalenol (DON), which threatens the health of humans and animals. However, some fungicides may stimulate F. graminearum to synthesize more DON under certain conditions. Until now, the fungicidal activity and enantioselective effect of prothioconazole enantiomers on DON production, transcriptome and metabolome of F. graminearum were unclear. The fungicidal activity of R-(-)-prothioconazole against F. graminearum was 9.12-17.73 times higher than that of S-(+)-prothioconazole under all conditions. Prothioconazole enantiomers can induce F. graminearum to synthesize more DON under 0.99 water activity (aw) and 30 °C, especially R-(-)-prothioconazole. The expression levels of TRI6, TRI10 and TRI101 under R-(-)-prothioconazole treatment were significantly higher than those under S-(+)-prothioconazole treatment. Most genes in glycolysis, pyruvate metabolism, the target of rapamycin (TOR) signaling transduction pathway and the cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) signaling transduction pathway showed higher expression levels under R-(-)-prothioconazole treatment than uner S-(+)-prothioconazole treatment and the control. The peroxisome pathway displayed higher transcriptional activity under S-(+)-prothioconazole treatment compared with R-(-)-prothioconazole and the control. Based on metabolomic data, R-(-)-prothioconazole can significantly influence phenylalanine metabolism, and no significantly enriched pathway was found under S-(+)-prothioconazole treatment. These results are helpful to understand the risk of prothioconazole enantiomers on DON production of F. graminearum and uncover the relevant underlying mechanisms of prothioconazole enantiomers.