Influence of gene modification in biological behaviors and responses of mouse lung telocytes to inflammation
Influence of gene modification in biological behaviors and responses of mouse lung telocytes to inflammation
复制标题
基因修饰对小鼠肺远程细胞生物学行为和炎症反应的影响
DOI：
10.1186/s12967-019-1870-y
复制
发表时间：
2019-05-15
影响因子：
7.4
通讯作者：
Wang, Xiangdong
中科院分区：
文献类型：
--
作者：
Song, Dongli;Xu, Menglin;Wang, Xiangdong
研究背景端细胞在维持器官/组织功能和预防器官损伤中起着重要作用。然而，由于分离、鉴定和稳定性的困难，使用初级端细胞研究端细胞功能存在很大的挑战。本研究旨在通过基因修饰构建小鼠肺端细胞连续系，并研究其生物学行为及对炎症反应的影响。用猴空泡病毒40小和大T抗原（SV 40）转化原代端细胞。以原代肺端细胞为对照，对转基因端细胞的生物学特性、行为形态和增殖进行动态观察。结果第5、10、30、50代转基因终末细胞均出现终足，并呈CD 34和ckit阳性。在celliq的监测下，端细胞系也观察到多种细胞形态，细胞增殖增强。结论成功构建了小鼠肺端细胞系，该细胞系保持了原代端细胞的生物学特性和行为，并能对LPS诱导的炎症反应产生应答。因此，基因修饰的肺端细胞，Telocyte Line，将为研究人员提供一个细胞工具，探索参与生理和病理状态的端细胞的作用和应用。
BackgroundTelocytes play key roles in maintenance of organ/tissue function and prevention of organ injury. However, there are great challenges to investigate telocytes functions using primary telocytes, due to the difficulties of isolation, identification, and stability. The present study aims at constructing continuous cell strain of mouse lung telocyte cell line with stable characters by gene modification and investigating biological behaviors and responses of gene-modified telocytes to inflammation.MethodsMouse primary lung telocytes were isolated and identified using immune-labeling markers and immunoelectron microscopy. Primary telocytes were transformed with Simian vacuolating virus 40 small and large T antigen (SV40). Biological characters, behaviors morphology, and proliferation of those gene-modified telocytes were defined and monitored dynamically for 50 generations, as compared with primary lung telocytes. Cell cycle of mouse primary lung telocytes or gene-modified telocytes was detected by flow cytometry.ResultsGene modified telocytes of generations 5, 10, 30 and 50 were observed with telopodes and also showed CD34 and ckit positive. Multiple cellular morphology were also observed on telocyte cell-line under monitor of celliq and enhanced cell proliferation were showed. SV40 transduction was also reduced apoptosis and increased the ratio of S and G2 phases in telocyte cell-line.ConclusionWe successfully constructed mouse lung telocyte cell-line which maintained the biological properties and behaviors as primary telocytes and could responses to inflammation induced by LPS. Thus, gene-modified lung telocytes, Telocyte Line, would provide a cell tool for researchers exploring the roles and applications of telocytes involved in physiological and pathological states in future.