Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy
Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy
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DOI：
10.1096/fj.13-239426
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发表时间：
2014-04-01
期刊：
影响因子：
4.8
通讯作者：
Peterson, Charlotte A.
中科院分区：
文献类型：
--
作者：
Fry, Christopher S.;Lee, Jonah D.;Peterson, Charlotte A.
我们当前研究的目的是确定卫星细胞对长期肌肉生长和维持的必要性。我们使用转基因Pax7-DTA小鼠模型，允许在他莫昔芬处理下有条件地消耗bb0 - 90%的卫星细胞。增效消融手术，其中去除增效肌肉使足底功能负荷过重，用于刺激强健肥厚。超负荷8周后，卫星细胞耗尽的肌肉表现出细胞外基质（ECM）的积累和成纤维细胞的扩张，导致足底比力降低。虽然早期的生长反应是正常的，但通过肌肉湿重和纤维横截面积测量，肥大的衰减发生在卫星细胞枯竭的肌肉中。体外分离的原代肌源性祖细胞（MPCs）负调控成纤维细胞ECM mRNA的表达，表明激活的卫星细胞/MPCs在肌肉适应中的新作用。这些结果提供了卫星细胞在生长过程中调节肌肉环境的证据。-Fry, c.s, Lee， J. D, Jackson， J. R, Kirby， T. J, Stasko， S. A, Liu， H., Dupont-Versteegden， E. E., McCarthy， J. J, Peterson， C. A.肥大过程中激活的卫星细胞对肌纤维微环境的调节。
Our aim in the current study was to determine the necessity of satellite cells for long-term muscle growth and maintenance. We utilized a transgenic Pax7-DTA mouse model, allowing for the conditional depletion of > 90% of satellite cells with tamoxifen treatment. Synergist ablation surgery, where removal of synergist muscles places functional overload on the plantaris, was used to stimulate robust hypertrophy. Following 8 wk of overload, satellite cell-depleted muscle demonstrated an accumulation of extracellular matrix (ECM) and fibroblast expansion that resulted in reduced specific force of the plantaris. Although the early growth response was normal, an attenuation of hypertrophy measured by both muscle wet weight and fiber cross-sectional area occurred in satellite cell-depleted muscle. Isolated primary myogenic progenitor cells (MPCs) negatively regulated fibroblast ECM mRNA expression in vitro, suggesting a novel role for activated satellite cells/MPCs in muscle adaptation. These results provide evidence that satellite cells regulate the muscle environment during growth.-Fry, C. S., Lee, J. D., Jackson, J. R., Kirby, T. J., Stasko, S. A., Liu, H., Dupont-Versteegden, E. E., McCarthy, J. J., Peterson, C. A. Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy.