Effects of magnesium deficiency on magnesium uptake activity of rice root, evaluated using 28 Mg as a tracer
Effects of magnesium deficiency on magnesium uptake activity of rice root, evaluated using 28 Mg as a tracer
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DOI：
10.1007/s11104-014-2197-3
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发表时间：
2014-07
期刊：
影响因子：
4.9
通讯作者：
K. Tanoi;N. Kobayashi;T. Saito;N. Iwata;Risa Kamada;R. Iwata;H. Suzuki;A. Hirose;Y. Ohmae
中科院分区：
文献类型：
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作者：
K. Tanoi;N. Kobayashi;T. Saito;N. Iwata;Risa Kamada;R. Iwata;H. Suzuki;A. Hirose;Y. Ohmae
目的植物根系吸收镁的机制尚未完全阐明。特别是，很少有关于镁缺乏对镁吸收活性的影响的信息。镁吸收动力学研究是必要的，更好地了解镁吸收system.MethodsWe进行了示踪实验在水稻植株中使用28 Mg.ResultsMg吸收介导的高，低亲和力的运输系统。在缺镁条件下，高亲和力转运系统的Km值约为70 μM。缺镁促进了镁的吸收活性，而镁再供应5分钟后又下降到基础水平。诱导的吸收率被抑制离子载体处理，这表明一个能量依赖性的吸收系统是增强镁deficiency.ConclusionsThe镁吸收迅速变化，镁条件下，在水稻，揭示了由28镁示踪实验。这种技术预计将适用于镁吸收分析，特别是在突变体或其他线。
AimsThe mechanisms underlying magnesium (Mg) uptake by plant roots remain to be fully elucidated. In particular, there is little information about the effects of Mg deficiency on Mg uptake activity. A Mg uptake kinetic study is essential for better understanding the Mg uptake system.MethodsWe performed a Mg uptake tracer experiment in rice plants using28Mg.ResultsMg uptake was mediated by high- and low-affinity transport systems. TheKmvalue of the high-affinity transport system was approximately 70 μM under Mg-deficient conditions. The Mg uptake activity was promoted by Mg deficiency, which in turn fell to the basal level after 5- min of Mg resupply. The induced uptake rate was inhibited by ionophore treatment, suggesting that an energy-dependent uptake system is enhanced by Mg deficiency.ConclusionsThe Mg uptake changes rapidly with Mg conditions in rice, as revealed by a28Mg tracer experiment. This technique is expected to be applicable for Mg uptake analyses, particularly in mutants or other lines.