Assessment of exercise-induced stress by automated measurement of salivary cortisol concentrations within the circadian rhythm in Japanese female long-distance runners
Assessment of exercise-induced stress by automated measurement of salivary cortisol concentrations within the circadian rhythm in Japanese female long-distance runners
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DOI：
10.1186/s40798-020-00269-4
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发表时间：
2020-08-17
影响因子：
4.6
通讯作者：
Murakami, Masami
中科院分区：
文献类型：
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作者：
Ushiki, Kazumi;Tsunekawa, Katsuhiko;Murakami, Masami
研究背景过度训练综合征是由长期过度紧张引起的，可导致运动员的运动成绩和皮质醇反应性下降。为了通过测量皮质醇浓度来评估运动应激，有必要在昼夜节律内连续收集唾液样本，并且使用电化学发光免疫测定法（ECLIA）的自动皮质醇测量可用于测量大量唾液样本。在这项研究中，我们评估了适当使用皮质醇为基础的运动应激评估的昼夜节律，这可能会诊断和预防过度训练综合征的运动员。方法收集54例健康志愿者的唾液和血清，用电化学发光法（ECLIA）测定唾液皮质醇浓度，并与酶联免疫吸附试验（ELISA）和血清皮质醇测定结果进行相关性分析。我们还连续收集了12名女性长跑运动员的唾液，连续2天，包括不同强度和类型的运动在清晨和下午，并使用ECLIA测量唾液皮质醇浓度。通过跑步速度、博格量表评分和运动引起的脉率变化率来测量跑步者的每种运动强度。结果ECLIA法测得的唾液皮质醇浓度与ELISA法测得的浓度（rho= 0.924，p < 0.001）和血清皮质醇浓度（rho= 0.591，p = 0.001）呈正相关。在长跑运动员中，唾液皮质醇的昼夜节律，包括清醒后的峰值和此后迅速下降，在两天的连续唾液采样检测。两天清晨运动后唾液皮质醇浓度的变化率均显著低于下午运动后（第1天，p= 0.002，第2天，p= 0.003）。在清晨运动中，唾液皮质醇浓度的变化率在第1天显著高于第2天（p= 0.034），类似于跑步速度的显著差异（p= 0.001）。结论基于ECLIA的唾液皮质醇自动检测方法能够检测运动员的昼夜节律，并比较不同时间（包括清晨）的运动应激强度，可能有助于预防过度训练综合征。
Background Overtraining syndrome, caused by prolonged excessive stress, results in reduced performance and cortisol responsiveness in athletes. It is necessary to collect saliva samples sequentially within circadian rhythm for assessing exercise stress by measuring cortisol concentrations, and automated cortisol measurements using electrochemiluminescence immunoassay (ECLIA) may be useful for measuring a large number of saliva samples. In this study, we evaluated the appropriate use of cortisol-based exercise stress assessment within the circadian rhythm, which may diagnose and prevent overtraining syndrome in athletes. Methods We collected saliva and sera from 54 healthy participants and analyzed the correlation between salivary cortisol concentrations measured by ECLIA and enzyme-linked immunosorbent assay (ELISA) or serum cortisol analysis. We also collected saliva continuously from 12 female long-distance runners on 2 consecutive days involving different intensities and types of exercise early in the morning and in the afternoon and measured salivary cortisol concentrations using ECLIA. Each exercise intensity of runners was measured by running velocities, Borg Scale score, and rate of change in the pulse rate by exercise. Results ECLIA-based salivary cortisol concentrations correlated positively with those detected by ELISA (rho= 0.924,p< 0.001) and serum cortisol (rho= 0.591,p= 0.001). In long-distance runners, circadian rhythm of salivary cortisol, including the peak after waking and the decrease promptly thereafter, were detected on both days by continuous saliva sampling. The rates of change in salivary cortisol concentrations were significantly lower after an early morning exercise than after an afternoon exercise on both days (day 1,p= 0.002, and day 2,p= 0.003). In the early morning exercise, the rate of change in salivary cortisol concentration was significantly higher on day 1 than on day 2 (p= 0.034), similar to a significant difference in running velocities (p= 0.001). Conclusions Our results suggest that automated ECLIA-based salivary cortisol measurements are able to detect the athletes' circadian rhythm and compare the exercise stress intensities at the same times on different days, even in the early morning, possibly leading to the prevention of overtraining syndrome.