A kinetic model of the co-operative binding of calcium and ADP to scallop (Argopecten irradians) heavy meromyosin.
A kinetic model of the co-operative binding of calcium and ADP to scallop (Argopecten irradians) heavy meromyosin.
复制标题
钙和 ADP 与扇贝（Argopecten irradians）重 meromyosin 协同结合的动力学模型。
DOI：
10.1042/bj20020099
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Geeves,MichaelA
中科院分区：
文献类型：
--
作者：
Nyitrai,Miklos;Szent-Gyorgyi,AndrewG;Geeves,MichaelA
Analysis of the kinetics of ATP and ADP binding to scallop (Argopecten irradians) heavy meromyosin (HMM) showed that the only calcium-dependent process is the rate of ADP release. At physiological ionic strength calcium accelerated ADP release about 20-fold. Notably in the absence of calcium only one ADP bound HMM, with an affinity of 0.5–1μM. The second nucleotide site remained unoccupied at up to 50μM ADP yet could bind ATP rapidly. The calcium dependence of ADP-release rates showed that calcium binds co-operatively to scallop HMM with an affinity of 0.78μM and a Hill coefficient of 1.9. Detailed interpretation of the data suggests that HMM exists in equilibrium between the on and off states and that calcium and ADP modulate the equilibrium between the two states. The on state is favoured in the presence of calcium and in the absence of both calcium and nucleotide. The off state is favoured by ADP (or ADP·Pi) in the absence of calcium. A detailed co-operative model of the interaction of ADP and calcium with HMM is presented.
登录
查看更多内容
镁离子依赖性腺苷三磷酸酶反应过程中肌球蛋白产物复合物和产物释放步骤的表征。
影响因子：
4.1
作者：
Clive R. Bagshaw;D. Trentham
通讯作者：
D. Trentham
影响因子：
--
作者：
Kalabokis,VN;Szent-Györgyi,AG
通讯作者：
Szent-Györgyi,AG
DOI：
10.1016/0304-4165(87)90102-4
发表时间：
1987-08-13
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
HARRISON, SM;BERS, DM
通讯作者：
BERS, DM
通过钙结合肌球蛋白调节收缩。
影响因子：
3.8
作者：
Szent-Györgyi,AG
通讯作者：
Szent-Györgyi,AG
亚片段 1 和 actosubfragment 1 ATPase 的中间状态：重新评估机制。
影响因子：
2.9
作者：
K. Johnson;E. Taylor
通讯作者：
E. Taylor