PHENOTYPIC AND GENETIC-CHARACTERIZATION OF A UNIQUE LYMPHOCYTE-B DEFICIENCY IN STRAIN A/WYSNJ MICE
PHENOTYPIC AND GENETIC-CHARACTERIZATION OF A UNIQUE LYMPHOCYTE-B DEFICIENCY IN STRAIN A/WYSNJ MICE
复制标题
DOI：
10.1002/eji.1830210506
复制
发表时间：
1991-05-01
影响因子：
5.4
通讯作者：
HAYES, CE
中科院分区：
文献类型：
--
作者：
MILLER, DJ;HAYES, CE
通过诸如X连锁免疫缺陷（xid）突变等遗传模型，对B淋巴细胞发育的阐释已部分实现。我们在A/WySnJ品系小鼠中发现了一种独特的B淋巴细胞发育缺陷。我们使用单色和双色流式细胞术分析了A/J和A/WySnJ小鼠的淋巴细胞。与成年A/J小鼠相比，成年A/WySnJ小鼠存在严重的B细胞缺陷，这在脾脏、淋巴结、腹膜和外周血中都很明显。一项个体发生研究表明，一种抑制B淋巴细胞成熟或分化但不影响B淋巴细胞生成的发育缺陷是导致这种缺陷的原因。这种成熟缺陷阻碍了B220高/Ia（高）/表面IgM（低）B细胞的产生，并且在成年骨髓和新生脾脏中表现出来，但在成年脾脏中没有。Ly - 1 B细胞和外周T细胞显然都没有受到A/WySnJ缺陷的影响。在F1和F2小鼠中，B细胞免疫缺陷作为一种常染色体共显性性状分离。我们提出A/WySnJ小鼠存在一种新的遗传缺陷，阻碍了成年骨髓和新生脾脏中B细胞的分化。
The elucidation of B lymphocyte development has been partially achieved through genetic models such as the X-linked immunodeficiency (xid) mutation. We discovered a unique B lymphocyte developmental defect in strain A/WySnJ mice. We used single- and two-color flow cytometry to analyze lymphocytes from A/J and A/WySnJ mice. Adult A/WySnJ mice had asevere B cell deficiency, which was apparent in the spleen, lymph nodes, peritoneum and peripheral blood, compared to adult A/J mice. An ontogeny study revealed that a developmental defect, inhibiting B lymphocyte maturation or differentiation but not B lymphopoiesis, was responsible for the deficiency. This maturational defect blocked the production of B220hi/Ia(hi)/surface IgM(lo) B cells, and was manifested in the adult bone marrow and neonatal spleen, but not the adult spleen. Neither Ly-1 B cells nor peripheral T cells were apparently affected by the A/WySnJ defect. The B cell immunodeficiency segregated as an autosomal co-dominant trait in F1 and F2 mice. We propose that A/WySnJ mice have a novel genetic defect arresting B cell differentiation in adult bone marrow and neonatal spleen.