Two classes of endogenous small RNAs in Tetrahymena thermophila
Two classes of endogenous small RNAs in Tetrahymena thermophila
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DOI：
10.1101/gad.1377006
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发表时间：
2006-01-01
影响因子：
10.5
通讯作者：
Collins, K
中科院分区：
文献类型：
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作者：
Lee, SR;Collins, K
内源性小RNA在RNA干扰（RNAi）途径中起作用以引导RNA切割、翻译抑制或DNA或染色质的甲基化。在嗜热四膜虫中，发育调节的DNA消除由涉及类似于27-30个核苷酸（nt）的RNA的RNAi机制控制。在这里，我们描述了类似于27-30-nt RNA和类似于23-24-nt RNA的序列特征，代表了第二个RNAi途径。与23-24-nt相似的RNA以菌株特异性的方式积累，并以与预测基因反义的簇定位到基因组中。这些发现揭示了在单细胞T.嗜热菌，以前只在多细胞生物中证明的复杂性。
Endogenous small RNAs function in RNA interference (RNAi) pathways to guide RNA cleavage, translational repression, or methylation of DNA or chromatin. In Tetrahymena thermophila, developmentally regulated DNA elimination is governed by an RNAi mechanism involving similar to 27-30-nucleotide (nt) RNAs. Here we characterize the sequence features of the similar to 27-30-nt RNAs and a similar to 23-24-nt RNA class representing a second RNAi pathway. The similar to 23-24-nt RNAs accumulate strain-specifically manner and map to the genome in clusters that are antisense to predicted genes. These findings reveal the existence of distinct endogenous RNAi pathways in the unicellular T. thermophila, a complexity previously demonstrated only in multicellular organisms.