A polyphenol-rich cranberry extract protects from diet-induced obesity, insulin resistance and intestinal inflammation in association with increased Akkermansia spp. population in the gut microbiota of mice
A polyphenol-rich cranberry extract protects from diet-induced obesity, insulin resistance and intestinal inflammation in association with increased Akkermansia spp. population in the gut microbiota of mice
复制标题
DOI：
10.1136/gutjnl-2014-307142
复制
发表时间：
2015-06-01
期刊：
影响因子：
24.5
通讯作者：
Marette, Andre
中科院分区：
文献类型：
--
作者：
Anhe, Fernando F.;Roy, Denis;Marette, Andre
目的肥胖症和2型糖尿病（T2 D）患病率的增加表明常规治疗无法控制这些疾病。肠道微生物群已被提出作为饮食诱导的T2 D的病理生理学中的关键参与者。重要的是，蔓越莓（大果越桔艾顿）与许多有益健康的影响有关。我们的目的是研究蔓越莓提取物（CE）对高脂肪/高蔗糖（HFHS）喂养的小鼠的代谢影响，并确定其随之而来的抗糖尿病作用是否与肠道微生物群的调节有关。设计C57 BL/6 J小鼠喂养食物或HFHS饮食。HFHS喂养的小鼠每天用载体（水）或CE（200 mg/kg）管饲8周。肠道菌群的组成进行了评估，通过分析16 S rRNA基因序列与454焦磷酸sequencing.Results CE治疗发现，以减少HFHS诱导的体重增加和内脏肥胖。CE治疗还降低了肝脏重量和甘油三酯蓄积，并减弱了肝脏氧化应激和炎症。口服葡萄糖耐量试验期间，CE给药改善了胰岛素敏感性，如胰岛素耐受性改善、胰岛素抵抗的稳态模型评估降低和葡萄糖诱导的高胰岛素血症减少所示。CE治疗被发现降低肠道甘油三酯含量，减轻肠道炎症和氧化应激。有趣的是，CE治疗显着增加粘蛋白降解细菌阿克曼氏菌在我们的宏基因组samples.Conclusions CE发挥有益的代谢作用，通过改善HFHS饮食诱导的代谢综合征的功能，这是与阿克曼氏菌属的比例增加。人口
Objective The increasing prevalence of obesity and type 2 diabetes (T2D) demonstrates the failure of conventional treatments to curb these diseases. The gut microbiota has been put forward as a key player in the pathophysiology of diet-induced T2D. Importantly, cranberry (Vaccinium macrocarpon Aiton) is associated with a number of beneficial health effects. We aimed to investigate the metabolic impact of a cranberry extract (CE) on high fat/high sucrose (HFHS)-fed mice and to determine whether its consequent antidiabetic effects are related to modulations in the gut microbiota.Design C57BL/6J mice were fed either a chow or a HFHS diet. HFHS-fed mice were gavaged daily either with vehicle (water) or CE (200mg/kg) for 8weeks. The composition of the gut microbiota was assessed by analysing 16S rRNA gene sequences with 454 pyrosequencing.Results CE treatment was found to reduce HFHS-induced weight gain and visceral obesity. CE treatment also decreased liver weight and triglyceride accumulation in association with blunted hepatic oxidative stress and inflammation. CE administration improved insulin sensitivity, as revealed by improved insulin tolerance, lower homeostasis model assessment of insulin resistance and decreased glucose-induced hyperinsulinaemia during an oral glucose tolerance test. CE treatment was found to lower intestinal triglyceride content and to alleviate intestinal inflammation and oxidative stress. Interestingly, CE treatment markedly increased the proportion of the mucin-degrading bacterium Akkermansia in our metagenomic samples.Conclusions CE exerts beneficial metabolic effects through improving HFHS diet-induced features of the metabolic syndrome, which is associated with a proportional increase in Akkermansia spp. population.