Multifaceted analysis of nanotoxicity using primary cultured neurons
Multifaceted analysis of nanotoxicity using primary cultured neurons
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使用原代培养神经元对纳米毒性进行多方面分析
DOI：
10.1088/2632-959x/ac7cfd
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发表时间：
2022
期刊：
影响因子：
3
通讯作者：
Sakurai Takashi
中科院分区：
文献类型：
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作者：
Kamikubo Yuji;Yamana Tomohito;Inoue Yuriko;Sakurai Takashi
随着纳米技术的发展，已经生产出各种纳米材料，据报道，其中一些材料对几种类型的细胞、器官和环境具有不利影响。有人提出，一些小的纳米颗粒可以穿过血脑屏障并在大脑中积聚，这可能是脑部疾病的潜在原因。神经元细胞易受缺氧、营养不良、机械和氧化应激的影响。因此，准确评估纳米颗粒对神经元的毒性至关重要。在这份报告中，我们描述了一个原代培养协议，以评估纳米粒子对神经元的毒性，一个潜在的高通量的方法来评估细胞毒性，和一种方法来评估对神经元成熟的影响。本报告使用大鼠海马神经元评估了二氧化硅、氧化锌和铁纳米颗粒的毒性，这些神经元经常用于药理学和生理学研究。根据我们在本报告中报告的方法和方案，有可能通过使用其他脑区（大脑皮层、小脑、丘脑等）的原代培养物来评估对各种神经元的纳米毒性，脊髓和周围神经。
Various nanomaterials have been produced with the development of nanotechnology, some of which have been reported to have adverse effects on several types of cells, organs, and the environment. It has been suggested that some small nanoparticles can cross the blood-brain barrier and accumulate in the brain, which may be a potential cause of brain diseases. Neuronal cells are vulnerable to hypoxia, hypotrophy, and mechanical and oxidative stress. Therefore, it is essential to assess the toxicity of nanoparticles to neurons accurately. In this report, we describe a primary culture protocol to evaluate the toxicity of nanoparticles on neurons, a potential high-throughput method for assessing the cytotoxicity, and a method for evaluating the effect on neuronal maturation. This report assessed the toxicity of silicon dioxide, zinc oxide, and iron nanoparticles using rat hippocampal neurons, which are used frequently in pharmacological and physiological studies. Based on the methods and protocols we reported in this report, it may be possible to evaluate nanotoxicity to various neurons by using primary cultures of other brain regions (cerebral cortex, cerebellum, thalamus, etc.), spinal cord, and peripheral nerves.