PCR as a confirmatory technique for laboratory diagnosis of malaria

PCR as a confirmatory technique for laboratory diagnosis of malaria
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DOI:
10.1128/jcm.44.3.1087-1089.2006
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发表时间:
2006-03-01
影响因子:
9.4
通讯作者:
da Silva, AJ
da Silva, AJ
中科院分区:
医学2区
文献类型:
--
作者:
Johnston, SP;Pieniazek, NJ;da Silva, AJ

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我们比较了巢式PCR法和吉氏染色血膜镜检对血液标本中疟原虫的检测和鉴定。PCR比镜检更敏感,在镜检不明确的情况下能够在种水平上识别疟原虫。
We compared a nested PCR assay and microscopic examination of Giemsa-stained blood films for detection and identification of Plasmodium spp. in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal.