Two-Week versus Six-Month Sampling Interval in a Short-Term Natural History Study of Oral HPV Infection in an HIV-Positive Cohort
Two-Week versus Six-Month Sampling Interval in a Short-Term Natural History Study of Oral HPV Infection in an HIV-Positive Cohort
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DOI：
10.1371/journal.pone.0011918
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发表时间：
2010-07-30
期刊：
影响因子：
3.7
通讯作者：
Gillison, Maura
中科院分区：
文献类型：
--
作者：
Fakhry, Carole;Sugar, Elizabeth;Gillison, Maura
背景：每隔6个月检测一次口腔HPV感染，并与在其间的几个月中在HIV阳性队列中每两周检测一次的口腔HPV感染进行比较，以阐明通过抽样间隔引入自然历史研究中的系统性偏差。方法：从HIV阳性队列(n=112)连续6个月每两周收集14份口腔含漱液样本，并评估37种HPV类型的存在。在第1次至第14次访问中检测到特定类型HPV的累积概率被确定为在第1次访问和第14次访问中被归类为持续、新检测、清除或不存在的感染的函数。结果：口腔HPV感染在两周和六个月的平均时点感染率相似(45%比47%，p=0.52)，但累积感染率高于前者(82%比53%，p=0.52)。
Background: Oral HPV infections detected six-months apart were compared to those detected bi-weekly, in an HIV-positive cohort, during the intervening months to elucidate systematic biases introduced into natural history studies by sampling interval.Methods: Fourteen consecutive oral rinse samples were collected every two weeks for six months from an HIV-positive cohort (n = 112) and evaluated for the presence of 37 HPV types. The cumulative probability of type-specific HPV detection at visits 1 through 14 was determined as a function of infection categorized at visits 1 and 14 as persistent, newly detected, cleared or absent. Transition models were used to evaluate the effect of HPV viral load (measured by RT-PCR for HPV 16, 18, 31, 33, 35) on infection persistence.Results: The average point prevalence of oral HPV infection was similar at two-week and six-month sampling intervals (45% vs. 47%, p = 0.52), but cumulative prevalence was higher with the former (82% vs. 53%, p