Radioprotection of Bone Marrow Hematopoiesis by CpG-oligodeoxynucleotides Administered to Mice after Total-body Irradiation
Radioprotection of Bone Marrow Hematopoiesis by CpG-oligodeoxynucleotides Administered to Mice after Total-body Irradiation
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全身照射后给予小鼠 CpG 寡脱氧核苷酸对骨髓造血的辐射保护
DOI：
10.1269/jrr.10098
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发表时间：
2011-11-01
影响因子：
2
通讯作者：
Cai, Jianming
中科院分区：
文献类型：
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作者：
Zhang, Chao;Lin, Jing;Cai, Jianming
CpG-寡脱氧核苷酸 (ODN) 是细菌 DNA 的合成类似物，由于它能激活适应性免疫系统和先天免疫系统的细胞而引起了人们的关注。在本研究中，我们研究了全身照射 (TBI) 后施用 CpG-ODN 是否具有放射防护作用。 TBI后30分钟、24小时和48小时内通过腹膜内注射(i.p)用50μg CpG-ODN治疗小鼠。我们的结果表明，不同程度的 TBI 的存活率均有所提高。计算得出的剂量减少因子 (DRF) 为 1.2。骨髓细胞计数和骨髓组织学检查表明CpG-ODN最大限度地减少了TBI引起的骨髓损伤。白细胞（WBC）计数、外源性（CFU-S）和内源性（endoCFU-S）集落形成单位脾脏计数数据表明，CpG-ODN可减轻原始造血干细胞损伤并在TBI后重建造血功能。因此，我们认为CpG-ODN有可能有助于提高存活率和限制TBI引起的骨髓抑制。
CpG-oligodeoxynucleotide (ODN), a synthetic analog of bacteria DNA, has attracted attention because it activates cells of an adaptive immune system and the innate immune system. In this study, we investigated whether CpG-ODN has radioprotective effects, when administered after total-body irradiation (TBI). Mice were treated with 50 mu g CpG-ODN via intraperitoneal injection (i.p) within 30 min, 24 h and 48 h after TBI. Our results showed that the survival rate was enhanced at various levels of TBI. The calculated dose reduction factor (DRF) was 1.2. Bone marrow cell count and bone marrow histological examination indicated that CpG-ODN minimized the bone marrow damage induced by TBI. The data of the white blood cell (WBC) count, exogenous (CFU-S) and endogenous (endoCFU-S) colony forming unit-spleen count demonstrated that CpG-ODN reduced primitive hematopoietic stem cells damage and reconstituted hematopoiesis after TBI. Thus, we suggested that CpG-ODN had the potential to contribute to the improvement of the survival rate and limitation of myelosuppression induced by TBI.