Phosphorus deficiency in Lupinus albus - Altered lateral root development and enhanced expression of phosphoenolpyruvate carboxylase
Phosphorus deficiency in Lupinus albus - Altered lateral root development and enhanced expression of phosphoenolpyruvate carboxylase
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DOI：
10.1104/pp.112.1.31
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发表时间：
1996-09-01
期刊：
影响因子：
7.4
通讯作者：
Allan, DL
中科院分区：
文献类型：
--
作者：
Johnson, JF;Vance, CP;Allan, DL
4.1.1.31研究了白色羽扇豆（Lupinus albus L.）用1 mM P（+P处理）或不用P（-P处理）生长。+ P处理的植物开始较少的丛生三级分生组织和这些分生组织的出现被推迟与-P处理的植物相比。蛋白质根区可以确定在两个P处理出苗后9天。PEPC mRNA的量，PEPC比活性，和酶蛋白在蛋白质类根比在正常根开始在10，12，和14天后，分别。PEPC mRNA、PEPC酶和PEPC比活性的增加表明该酶部分处于转录调节下。根系分泌物中有机酸的恢复与PEPC比活性的增加一致。- P-处理的植物渗出40-，20-，和Ei倍多柠檬酸，苹果酸，琥珀酸，分别比+P-处理的植物。所提供的数据支持这一假设，即白色羽扇豆协调调节蛋白质根的发育，PEPC的转录调节，和有机酸的生物合成的分泌响应磷缺乏。
The development of clustered tertiary lateral roots (proteoid roots) and the expression of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) in roots were studied in white lupin (Lupinus albus L.) grown with either 1 mM P (+P-treated) or without P (-P-treated). The +P-treated plants initiated fewer clustered tertiary meristems and the emergence of these meristems was delayed compared with -P-treated plants. Proteoid root zones could be identified 9 d after emergence in both P treatments. Amounts of PEPC mRNA, PEPC specific activity, and enzyme protein were greater in proteoid roots than in normal roots beginning at 10, 12, and 14 d after emergence, respectively. The increases in PEPC mRNA, PEPC enzyme, and PEPC specific activity suggest that this enzyme is in part under transcriptional regulation. Recovery of organic acids from root exudates coincided with the increases in PEPC specific activity. The -P-treated plants exuded 40-, 20-, and Ei-fold more citrate, malate, and succinate, respectively, than did +P-treated plants. Data presented support the hypothesis that white lupin has concerted regulation of proteoid root development, transcriptional regulation of PEPC, and biosynthesis of organic acids for exudation in response to P deficiency.